ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Priyanka Pal1, Vishnu Prasad Shenoy1 and Promukh Bhattacharya2
1Department of Microbiology, Kasturba Medical College Manipal, Manipal Academy of Higher Education, Manipal, Karnataka, India.
2Department of Microbiology, SRIMS & Sanaka Hospitals, Durgapur, West Bengal, India.
Article Number: 8106 | © The Author(s). 2023
J Pure Appl Microbiol. 2023;17(1):127-131.
Received: 16 September 2022 | Accepted: 29 November 2022 | Published online: 05 January 2023
Issue online: March 2023

Sputum microscopy is the most important tool for the detection of Mycobacterium tuberculosis in peripheral laboratories. Sodium hypochlorite concentration technique prior to sputum microscopy may improve the detection of AFB bacilli over direct Ziehl Neelsen’s staining method. A prospective study was conducted at Kasturba Medical College, Manipal. Consecutive sputum samples were collected for 3 months. ZN stained smears were made directly of fresh specimens and of specimens that were processed with 3.5% sodium hypochlorite (NaOCl), from each patient. Then, ZN stained smears were observed under light microscopy. A total of 239 samples were collected. The yield of positive smears with ZN staining after NaOCl centrifugation was higher than that with microscopy after direct ZN stain. The percentage increase yield of ZN staining after NaOCl centrifugation over direct ZN staining was 3.3% (8/239). Bleach centrifugation can help in increasing the yield of positivity in sputum smear.


Ziehl-Neelsen Staining, Sputum, Mycobacterium tuberculosis, Sodium Hypochlorite

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© The Author(s) 2023. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.