ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Herlina Septika Indahsari1, Asmi Citra Malina A.R. Tassakka2, Eko Nurcahya Dewi3, Mochammad Yuwono4 and Eko Agus Suyono1
1Faculty of Biology, Universitas Gadjah Mada, Jl. Teknika Selatan, Sleman 55281, Yogyakarta, Indonesia.
2Center of Excellence for Development & Utilization of Seaweeds, Faculty of Marine Science and Fisheries, Universitas Hasanuddin, Jl. Perintis Kemerdekaan Km. 10, Tamalanrea, Makassar 90245, South Sulawesi, Indonesia.
3Faculty of Fisheries and Marine Sciences, Universitas Diponegoro, Jl. Prof. Soedarto, SH, Semarang 50275, Central Java, Indonesia.
4Faculty of Pharmacy, Universitas Airlangga, C-campus, Mulyorejo, Surabaya 60115, East Java, Indonesia.
Article Number: 7887 | © The Author(s). 2022
J Pure Appl Microbiol. 2022;16(4):2901-2911. https://doi.org/10.22207/JPAM.16.4.65
Received: 07 June 2022 | Accepted: 28 October 2022 | Published online: 01 December 2022
Issue online: December 2022
Abstract

Euglena sp. is a freshwater microalga that produces useful metabolites in its biomass. The cultivation with oxidative stress treatments, such as salinity, plays a major role in maintaining the optimal cellular metabolic rate for the optimized growth of Euglena sp. for the production of lipids for biodiesel as well as carotenoids and chlorophyll, which are cell defense pigments that are beneficial for health. A bioflocculation method that uses microalgal flocculants, such as Skeletonema sp., is an alternative harvesting technique that is cost and energy saving. The effect of salinity and bioflocculation treatment on freshwater microalgae has not been widely studied. Therefore, this research determined the effect mechanism of salinity and bioflocculation in the production of lipids, chlorophyll, and carotenoids in Euglena sp. with Skeletonema sp. as a bioflocculant. In this research, the cultivation of Euglena sp. was carried out in Cramer–Myers medium for seven days under salinity treatments of 5, 10, 20, and 0 g/L, and the cultivation of Skeletonema sp. was performed for eight days in F/2 medium with modified silicate removal. Bioflocculation was performed by mixing Euglena sp. and Skeletonema sp. at ratios of 1:1, 1:0.5, and 1:0.25. The research results showed that salinity treatment, in general, succeeded in increasing the growth and production of lipid, chlorophyll, and carotenoid metabolites. The addition of Skeletonema sp. to the culture of Euglena sp. increased the precipitation percentage. A high mixing ratio increased the lipid level but decreased those of chlorophyll and carotenoid metabolites.

Keywords

Salinity, Bioflocculation, Euglena sp., Skeletonema sp.

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© The Author(s) 2022. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.