ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Nuniek Ina Ratnaningtyas1 , Fajar Husen2, Dalia Sukmawati3, Eko Setio Wibowo1, Arif Rahman Hikam1 and Adil Aksoy4
1Faculty of Biology, Jenderal Soedirman University (UNSOED), Purwokerto, 53122, Indonesia.
2Medical Laboratory of Technology, Bina Cipta Husada College of Health Science (STIKes), Purwokerto, 53144, Indonesia.
3Biology Department, 9th Floor Hasyim Building of Ashari, Faculty of Mathematics and Natural Sciences, Jakarta State University (UNJ), Jakarta, 13220, Indonesia.
4Laboratory Veterinary Science, Eskil Vocation of High School, Aksaray University, Aksaray, 68800, Turkey.
Article Number: 8018 | © The Author(s). 2022
J Pure Appl Microbiol. 2022;16(4):2764-2774.
Received: 06 August 2022 | Accepted: 17 October 2022 | Published online: 14 November 2022
Issue online: December 2022

Coprinus comatus (O.F. Mull.) Pers. is a medicinal and edible mushroom containing bioactive compounds known as antidiabetic and antioxidant agents. The pancreatic β-cells were sensitive to reactive oxygen species (ROS) attacks, which can cause necrosis and disrupt insulin production. Therefore, this research aimed to evaluate the C. comatus ethyl acetate extract for antidiabetic and antioxidant to decrease fasting blood glucose, dipeptidyl peptidase-4 enzyme (DPP-4) levels, and increase glutathione peroxidase (GPx). Subsequently, 24 male Wistar rats were divided into six groups, namely 2-5 streptozotocin (STZ)-induced 45 mg/kg, 1/HC (without any treatment), 2/NC (negative control), 3/PC (administered metformin 45 mg/kg Body weight (BW), 4-6 (T1: administered 250 mg, T2: administered 500 mg, and T3: administered 750 mg/kg BW extract). The parameters were analyzed using ANOVA, followed by Duncan’s multiple range test. According to the results, the extract significantly decreased DPP-4 levels, blood glucose and increased GPx (p ≤ 0.05). The 500 mg extract effectively reduced blood glucose levels and DPP-4 enzyme as well as increased levels of GPx.


Antidiabetic, Coprinus comatus, Enzymatic Antioxidant, Medicinal Mushroom, Reactive Oxygen Species

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© The Author(s) 2022. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.