ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Ramya Sivaramakrishnan, K.V. Leela, R. Sujith , Shweta Naik and Sneha Priya
Department of Microbiology, SRM Medical College Hospital and Research Centre, Faculty of Medicine and Health Sciences, SRM Institute of Science and Technology, SRM Nagar, Kattankulathur, Chenalpattu District, Tamil Nadu, India.
Article Number: 7278 | © The Author(s). 2022
J Pure Appl Microbiol. 2022;16(4):2669-2678. https://doi.org/10.22207/JPAM.16.4.36
Received: 26 August 2021 | Accepted: 17 August 2022 | Published online: 07 November 2022
Issue online: December 2022
Abstract

Klebsiella is a pathogen that causes a significantly high number of community-acquired and hospital-acquired infections, with infections being one of the leading causes of death in ICU patients worldwide due to increasing antibiotic-resistance and a lack of therapeutic options. A total of 230 Klebsiella spp. were collected from various clinical samples. After initial identification, the drug-resistant strain was subjected to standard Clinical Laboratory and Standards Institute methods such as Kirby–Bauer disc diffusion. All isolates were screened and confirmed for ESBL/AmpC β-lactamase/carbapenemase production. The isolated Klebsiella spp. were found to be K. pneumonia (89%), K. oxytoca (6.5%), and K. aerogenes (4.5%). Among the 230 isolates, 80 (34.7%) isolates were found to be ESBL producers via screening; of these, 53 (23.5%) were verified by a confirmatory test. Moreover, 115 isolates (50%) were screened as AmpC producers; of these, 23 isolates (10%) were verified by a confirmatory test. Carbapenemase producers accounted for 69 (30%) isolates, identified by screening; 25 (10.86%) were verified by a confirmatory test. ESBL producers accounted for the majority of Klebsiella spp. isolates, followed by carbapenem and AmpC producing strains.

Keywords

Klebsiella, Antibiotic resistance, ESBL ,Amp C β-lactamase, Carbapenemase

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