ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Smeera Thomas1,2, Greena Joshy2 and Kavitha Subbiah1
1Department of Biotechnology, Karunya Institute of Technology and Sciences (Deemed-to-be University), Karunya Nagar, Coimbatore, Tamil Nadu, India.
2Department of Biotechnology, Sahrdaya College of Engineering and Technology, Kodakara, Thrissur, Kerala, India.
Article Number: 7748 | © The Author(s). 2022
J Pure Appl Microbiol. 2022;16(4):2580-2591.
Received: 07 April 2022 | Accepted: 02 September 2022 | Published online: 27 October 2022
Issue online: December 2022

Biosurfactants were found to be a suitable candidate for environment clean-up and as an alternative to conventional treatment systems. In the present study, a polyaromatic hydrocarbon degrading organism Bacillus halotolerans was screened for its ability to produce biosurfactant during hydrocarbon degradation. The bio-surfactant activity of the organism was screened by using various methods like oil spreading technique, emulsification assay, foam height analysis and parafilm-M test. The design expert software was used to optimize the suitable conditions for the production of biosurfactants. The optimum conditions were determined as pH 6, Chrysene-100 ppm, nitrogen source -1000 ppm and at 144 hrs using the design of experiments. The organism showed good oil degradation capacity and maximum growth was achieved in 6 days. Biosurfactant obtained from the biodegradation medium was confirmed to be lipopeptide using TLC, FTIR and GCMS.


Bacillus halotolerans, Biosurfactant, Lipopeptides, Bioremediation, Hydrocarbons

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