ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Senthamarai Manogaran1 , Kannan Kilavan Packiam1, Vijayakumar Lakshmi Narayanan2, Chadhurthika Krishnamurthy1, Devi Vijayarangam1 and Moni Philip Jacob Kizhakedathil1
1Department of Biotechnology, Bannari Amman Institute of Technology, Sathyamangalam, Erode – 638 401, Tamil Nadu India.
2Department of Pharmaceutical Technology, Hindusthan Institute of Technology, Valley Campus, Coimbatore – 641 032, Tamil Nadu, India.
Article Number: 7716 | © The Author(s). 2022
J Pure Appl Microbiol. 2022;16(4):2477-2487. https://doi.org/10.22207/JPAM.16.4.07
Received: 28 March 2022 | Accepted: 28 June 2022 | Published online: 04 October 2022
Issue online: December 2022
Abstract

Lovastatin is a naturally produced 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductase enzyme inhibitor- used for treating hypercholesterolemia. It was the first statin drug which was approved by the United States Food and Drug Administration (USFDA). In the current study, endophytic fungus Fusarium nectrioides (MH173849) isolated from Euphorbia hirta L. was used for the production of lovastatin. Four different culture media indicated as M1, M2, M3 and M4 were used for the initial production of lovastatin. Liquid cheese whey was used as nitrogen source. Growth morphology of fungi was investigated using Scanning Electron Microscopy analysis. Also, parameters like temperature, pH, inoculum size, incubation time, and RPM were optimized for the obtaining highest lovastatin production. Among the four media, M4 was found to produce the maximum concentration of lovastatin. Parameters such as temperature of 28°C, pH 6, RPM – 180 rpm and inoculum size of 5 x107 spores/mL were optimal for the production of lovastatin by F. nectrioides (MH173849).

Keywords

Fusarium nectrioides, Lovastatin, Endophytic Fungus, Euphorbia hirta (Linn), Liquid Cheese Whey, Optimization

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