ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
S.J. Akshatha and Manjula Ishwara Kalyani
Department of Microbiology, Mangalore University, Jnana Kaveri, Chikka Aluvara, Kodagu, Karnataka, India.
Article Number: 7898 | © The Author(s). 2022
J Pure Appl Microbiol. 2022;16(4):2458-2476. https://doi.org/10.22207/JPAM.16.4.06
Received: 14 June 2022 | Accepted: 16 August 2022 | Published online: 04 October 2022
Issue online: December 2022
Abstract

Among actinobacteria, the genus Streptomyces are found in abundance in specific soil environments. Streptomyces are cultivable using Streptomyces-specific media, including starch casein, yeast extract, or ISP 2 media. Streptomyces isolates can be identified based on their macroscopic culture morphology and microscopic observations, and can be taxonomically placed within the Streptomyces genus. In the present study, mangrove soil samples collected from the coast of Mangalore harboring a multitude of microorganisms were enriched with calcium carbonate and pre-heated to isolate Streptomyces organisms. Cultures were quantified in colony forming units and their diversity was evaluated based on phenotypic features, enzyme hydrolysis, biochemical testing, and antibiotic sensitivity tests. The cross streaking method was used to select Streptomyces isolates, which were then further subjected to intracellular buffer extraction and evaluated against test organisms to determine their antibacterial efficacy. This study highlights the occurrence of prominent Streptomyces species with effective antibacterial activity in a unique environmental habitat of mangrove soil on the Mangalore coast.

Keywords

Mangrove-soil, Streptomyces, Slide Culturing, Antibacterial Activity, Cross Streak, Intracellular Extraction

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© The Author(s) 2022. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.