ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Fahad Abdullah Al-Dhabaan
Department of Biology, Science and Humanities College, Shaqra University, Al-Quwayiyah, Saudi Arabia.
Article Number: 8030 | © The Author(s). 2022
J Pure Appl Microbiol. 2022;16(4):2425-2438. https://doi.org/10.22207/JPAM.16.4.03
Received: 16 August 2022 | Accepted: 17 September 2022 | Published online: 28 September 2022
Issue online: December 2022
Abstract

Forty streptomycetes were isolated from the soils of farms in Riyadh. Only three isolates (St-2, St-9, and St-25) exhibited cellulolytic-ligninolytic activity, with the St-9 isolate exhibiting the highest activity and identified as Streptomyces lazureus. The optimum environmental and nutritional conditions for maximum cellulolytic-ligninolytic activity were determined as fermentation batch of pH of 7.5, inoculum size of 200 µL of bacterial suspension, incubation period of 7 d, and incubation temperature of 30°C. In addition, the fermentation batch contained peptone and yeast extract as the best nitrogen and carbon sources, respectively. Cellulase and ligninase were purified via gel filtration column chromatography. The accumulated end-product of the fermentation process was glucose powder, which was subjected to a partial characterization process. The glucose powder appeared white, melted at 146°C, was highly soluble in water, slightly soluble in ethanol, and insoluble in ethyl ether. The glucose solution appeared clear without precipitates and had a low electric conductivity of 15 µS.cm-1.

Keywords

Biodegradation, Cellulose, Glucose, Ligninase, Streptomycetes

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