The Chromobacterium violaceum is a gram-negative facultative anaerobic bacterium that is known to cause human infections in lungs, liver, brain, spleen lymph nodes and urinary tract. It has Acyl Homoserine Lactone (AHL) regulated virulence features like violacein pigment production, swarming motility, biofilm formation and haemolysis. Bacterial pathogens form biofilms in natural as well as medical implants due to a complex signalling – “Quorum Sensing” (QS). QS builds an interaction among the cells, which increases the proliferation and mechanisms necessary for invasion into the host. Instead of using only bactericidal agents for infection control, suppression of QS by Quorum Quenching agents (QQ) can overcome limitations of currently used antimicrobial substances. In the present study biogenic silver nanoparticles (BSNPs) synthesized from selected five plant extracts were screened against Chromobacterium violaceum MCC 4212 for QQ potential. Biofilm inhibition of 91.8% and dispersal of 81.33% was found to be exhibited by BSNPsmade from extracts of Garcinia and Trachyspermum. Swarming nature was inhibited by 66% while there was complete inhibition of haemolysis by BSNPs. Therefore, the BSNPs synthesized were found potential to control the pathogenicity of C. violaceum 4212 as an antibiofilm agent.
Biogenic Silver Nanoparticles, Chromobacterium Violaceum, Quorum Sensing Inhibition, Violacein, Antibiofilm
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