ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access

Balqees Yahya Najm1 , Sarab H. Khaleel2 and Hala Mahmmad Majeed3

1Molecular Genetics, Basic Science Department, Medicine College, Ibn Sina University of Medical and Pharmaceutical, Baghdad, Iraq.
2Cytogenetic, Basic Science Department, Medicine College, Ibn Sina University of Medical and Pharmaceutical, Baghdad, Iraq.
3Microbiology, Basic Science Department, Medicine College, Ibn Sina University of Medical and Pharmaceutical, Baghdad, Iraq.
Article Number: 7842 | © The Author(s). 2022
J Pure Appl Microbiol. 2022;16(3):2165-2172. https://doi.org/10.22207/JPAM.16.3.76
Received: 16 May 2022 | Accepted: 19 August 2022 | Published online: 31 August 2022
Issue online: September 2022
Abstract

Raw milk is a nutrient-rich food that is considered a high-quality nutritional medium for many microorganism, including Escherichia coli. The aim of the present work was the diagnosis, by molecular methods, of Shiga toxins produced by E. coli strains isolated from cow milk samples collected from different farms in Al-Mahmmodia, Al yoosifya, Al lattiffya, Al howasha, and Arab Jboor in the government of Baghdad during the summer season. Milk samples were incubated in media for bacterial isolation. Isolates were identified using Gram staining and biochemical tests. Out of 50 samples, 15 (30%) showed the presence of E. coli. To confirm the identity of the isolates, their 16S rRNA genes were amplified using specific primers. The results showed that all isolates were E. coli. Shiga toxin-producing E. coli (STEC) were detected among the samples. The prevalence of stx1 genes was higher than that of stx2 among them. No STECs were found among six of the sample isolates, and none of these isolates was positive for stx1 and stx2. SDS-PAGE was used to determine the molecular weight of the toxin, and four selected E. coli bacteria producing Shiga-like toxins showed a clear band of approximately 70 kDa.

Keywords

E. coli, Shiga Toxin, PCR, Milk Samples

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© The Author(s) 2022. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.