ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Archana Singh, Enketeswara Subudhi , Rajesh Kumar Sahoo, Mahendra Gaur and Sangita Dixit
Centre for Biotechnology, School of Pharmaceutical Sciences, Siksha ‘O’ Anusandhan (Deemed to be University), Bhubaneswar – 751 030, Odisha, India.
Article Number: 7818 | © The Author(s). 2022
J Pure Appl Microbiol. 2022;16(3):2131-2142. https://doi.org/10.22207/JPAM.16.3.70
Received: 05 May 2022 | Accepted: 08 July 2022 | Published online: 30 August 2022
Issue online: September 2022
Abstract

Hot springs are the reservoirs of novel hyperthermophilic and often mesophilic bacteria that provide information about the prevailing community structure. Here we analyzed the metagenome profile based 16S rRNA amplicon sequencing of the three different springs from Deulajhari hot spring cluster, S1, S2 and S3, having a range of temperature (43°C to 65°C), pH (7.14 – 8.10) and variation in N, P, K, TOC, Salinity, COD and TDS. These thermal spring clusters are covered with the dense vegetation of Pandanus and continuously enriched by plant leaf debris, thus resulting in a high amount of total organic carbon (TOC). The number of phyla varied among the springs: 20 in S1 (43°C), 18 in S2 (55°C) and 24 in S3 (65°C) from the 16S rRNA data. Out of the reported phyla in each spring, the most abundant were Chloroflexi, Proteobacteria, Chlorobi and Acidobacteria, which correlated with the temperature gradient. Various metabolic pathways such as ABC transporters, Two-component system, Purine metabolism were most abundantly present in the S2 sample compared to the other two. The CCA analysis revealed the correlation between physiochemical parameters and their functional annotation. The present study establishes the relation between the physiological parameters and the structural distribution of microbiota along the temperature gradient.

Keywords

Deulajhari, 16S rRNA, Hot Spring, Illumina, Next-generation Sequencing

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