ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Qanat Mahmood Atiyea1 , Fatima Mustafa Al-najar1, Gulbahar F. Karim2 and Siham Sh. AL-Salihi3
1Department of Biology, College of Science, University of Tikrit, Tikrit, Iraq.
2Department of Basic Sciences, College of Nursing, University of Kirkuk, Kirkuk, Iraq.
3Department of Medical Laboratory Techniques, College of Health and Medical Techniques, Northern Technical University, Kirkuk, Iraq.
Article Number: 7738 | © The Author(s). 2022
J Pure Appl Microbiol. 2022;16(3):2072-2082. https://doi.org/10.22207/JPAM.16.3.62
Received: 05 April 2022 | Accepted: 25 July 2022 | Published online: 27 August 2022
Issue online: September 2022
Abstract

Antimicrobial therapy is frequently associated with the emergence of resistant bacteria with a high rate of morbidity and mortality worldwide. The present study was aimed at investigating the impact of a neodymium-doped yttrium aluminum (Nd:YAG) laser, and a static magnetic field (SMF) on cellular growth and DNA alteration in some clinical bacterial isolates. Samples from cutaneous wounds were collected by sterile cotton swabs from three elderly women admitted to Tikrit Teaching Hospital, Tikrit City, Iraq. Isolation and identification of Streptococcus agalactiae, Staphylococcus aureus, and Pseudomonas aeruginosa were carried out using cultural characteristics, microscopy, and biochemical tests. Three broth cultures were prepared for each of the test isolates. The first broth culture served as untreated control, the second was exposed to an Nd:YAG laser and the third was exposed to SMF. Colony counting was done on all the samples. DNA was extracted from the test bacteria and used to perform the RAPD-PCR assay. In contrast to the untreated control, the results showed that Nd:YAG laser radiation was more effective than SMF at inhibiting the cellular growth of the test isolates. Also, the radiation caused DNA alteration, which was established by decreased microbial growth, as well as the development of new bands and the loss of original bands. According to the findings of this study, the Nd:YAG laser is a promising technique for influencing the healing of infected cutaneous wounds. RAPD-PCR is also a useful biomarker assay for assessing the biological impact of laser radiation and SMF on bacteria.

Keywords

Nd:YAG Laser, Static Magnetic Field, RAPD-PCR, S. agalactiae, S. aureus, Pseudomonas aeruginosa

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© The Author(s) 2022. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.