ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Soha A. Alamoudi1, Ashjan F. Khalel2, Meshal A. Alghamdi3,Wafa A. Alshehri4 , Ghadeer K. Alsubeihi4, Soha A. Alsolmy4 and Moayad A. Hakeem5
1Biological Science Department, College of Science and Arts, King Abdulaziz University, Rabigh 21911, Saudi Arabia.
2Department of Biology, Faculty of Science, King Abdul-Aziz University, Jeddah, Saudi Arabia.
3Department of Biology, Faculty of Science, Albaha University, Albaha 1988, Saudi Arabia.
4Department of Biology, College of Science, University of Jeddah, Jeddah, Saudi Arabia.
5Ministry of Health, Makkah, Saudi Arabia.
Article Number: 7850 | © The Author(s). 2022
J Pure Appl Microbiol. 2022;16(3):2045-2054. https://doi.org/10.22207/JPAM.16.3.58
Received: 08 March 2022 | Accepted: 20 July 2022 | Published online: 24 August 2022
Issue online: September 2022
Abstract

Keratinolytic microorganisms are highly valuable for decomposition of poultry waste. This study aimed to isolate keratin-decomposing actinobacteria from poultry farm soils and examine their capacity to decompose feathers. Soil samples were placed in a basal medium with feather meal, which is a deposit of carbon and nitrogen. Nine actinobacterial strains were isolated. Actinobacteria were cultured in the media to show clear feather-decomposing potential. Actinobacterial strains were identified using 16S ribosomal RNA (rRNA) sequencing as being related to Streptomyces rochei AM8. Thus, the supernatant of S. rochei AM8 exhibited keratinolytic enzyme activity. Increased biodecomposition of feathers was recorded in a keratinase assay (0.782 U/mL) for separated cultures. The ability of the selected microorganisms to decompose feathers may be an effective biotechnological solution for managing feather waste from poultry.

Keywords

Feather-degrading Actinobacteria, Characterization, Identification, Keratinase, Feather Waste

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