ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
R. Lakshmanan1, Muhammad Musthafa Poyil2, D. Kalaimurugan1, P. Sivasankar1, K. Ponmurugan3 and S. Venkatesan1
1Department of Environmental Science, School of Life Sciences, Periyar University, Salem – 636 011, Tamil Nadu, India.
2Department of Basic Medical Sciences, College of Medicine, Prince Sattam bin Abdulaziz  University, Al-Kharj, 11942, Saudi Arabia.
3Department of Botany and Microbiology, College of Science, King Saud University, Riyadh – 11451, Saudi Arabia.
Article Number: 7146 | © The Author(s). 2022
J Pure Appl Microbiol. 2022;16(3):1998-2009. https://doi.org/10.22207/JPAM.16.3.50
Received: 01 July 2021 | Accepted: 15 May 2022 | Published online: 17 August 2022
Issue online: September 2022
Abstract

Plant growth-promoting rhizobacteria (PGPR) have proved to be an effective solution for enhancing growth of various plant species. Five different bacterial isolates extracted from rhizosphere soil were extensively studied for the production of indole acetic acid (IAA) and among those Bacillus safensis YKS2 strain was found to produce substantial quantities of IAA. B. safensis YKS2 strain was characterized and submitted to National Centre for Biotechnology Information (NCBI) (Gen Bank No. MH539636). Optimization of IAA production with varying pH and temperature revealed that IAA production was maximum at pH 7 and at a temperature of 37°C. The production of IAA was confirmed and quantified by Fourier-transform infrared spectroscopy (FTIR), Thin-layer chromatography (TLC), Gas chromatography-mass spectrometry (GC-MS). The PGPR inoculum showed significant (p<0.05) shoot increase (60.00 – 89.00%) and root increase (30.00 – 90.00%) relative to the controls in Vigna radiata. This study showed that IAA producing ability of B. safensis YKS2 can be used in the large-scale production of IAA for plant growth promotion.

Keywords

Rhizobacteria, Indole Acetic Acid, Bacillus safensis, Vigna Radiata, PGPR

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