ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
L. Sugantha Bharathy, B. Anu Monisha and K. Sathiyamurthy
Department of Biomedical Science, School of Biotechnology and Genetic Engineering, Bharathidasan University, Trichy – 620 024, Tamil Nadu, India.
Article Number: 7610 | © The Author(s). 2022
J Pure Appl Microbiol. 2022;16(3):1990-1997.
Received: 24 February 2022 | Accepted: 13 July 2022 | Published online: 17 August 2022
Issue online: September 2022

The objective of the study is to determine the antibiotic resistance and biofilm formation of Klebsiella pneumoniae in different surfaces. In this study, 47 K. pneumoniae strains were procured from K.A.P. Viswanatham Government Medical College, Trichy, Tamil Nadu. All the isolates were reconfirmed through biochemical reactions. Maximum resistance was observed against Ampicillin, Cefepime, Cefotaxime, Co-trimoxazole, Aztreonam, and Imipenem. The lowest resistance was noticed against Cefuroxime. Among these 85% strains exhibited multidrug resistance with 13% was carbapenem and 98% ESBL resistant strains. The biofilm formation of all the strains in different surfaces revealed that stainless steel surface found to be adhered high number of cells than in other surfaces. Similarly, the biofilm formation of strains grown with glucose in tryptic soya broth (TSB) enhanced adherence ability. It is concluded that presence of glucose or any sugar substrates enhances the biofilm formation thereby developing high resistance against different antibiotics. This condition is detrimental to human health and causes considerable concern.


Klebsiella pneumoniae, Antibiotic Resistance, Biofilm, Material Surfaces

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