ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
D. Danis Vijay1,2, S.H. Shifa Meharaj3, S. Jayanthi3 , A. Sujhithra4,7, R. Vidhya1, N. Meenakshi5 and Aruna Shanmuganathan5,6
1Department of Microbiology, Chettinad Hospital and Research Institute, Chettinad Academy of Research & Education, Kelambakkam – 603 103, Tamilnadu, India.
2Department of Microbiology, Karpaga Vinayaga Institute of Medical Sciences and Research Center. P.O, GST Road, Chinna Kolambakkam, Palayanoor, Maduranthakam – 603 308, Tamilnadu, India.
3Department of Microbiology, Panimalar Medical College Hospital & Research Institute, Varadhrajapuram, Poonamallee, Chennai – 600 123, Tamilnadu, India.
4Faculty of Allied Health Sciences, Department of Cardiology, Chettinad Hospital and Research Institute (CHRI), Chettinad Academy of Research and Education (CARE), Kelambakkam – 603 103, Tamilnadu, India.
5Department of Respiratory Medicine, Chettinad Hospital and Research Institute, Chettinad Academy of Research & Education, Kelambakkam – 603 103, Tamilnadu, India.
6Department of Respiratory Medicine, Karpaga Vinayaga Institute of Medical Sciences and Research Center. P.O, GST Road, Chinna Kolambakkam, Palayanoor, Maduranthakam- 603 308, Tamilnadu, India.
7Faculty of Allied Health Sciences, Department of Cardiology, Karpaga Vinayaga Institute of Medical Sciences and Research Center. P.O, GST Road, Chinna Kolambakkam, Palayanoor, Maduranthakam – 603308, Tamilnadu, India.
Article Number: 7595 | © The Author(s). 2022
J Pure Appl Microbiol. 2022;16(3):1953-1963. https://doi.org/10.22207/JPAM.16.3.42
Received: 04 February 2022 | Accepted: 08 May 2022 | Published online: 12 August 2022
Issue online: September 2022
Abstract

Tuberculosis (TB) is a serious and important public health issue to be addressed in India. Timely diagnosis of the drug resistance in tuberculosis is essential to ensure and initiate appropriate therapy. The detection of drug resistant Mycobacterium tuberculosis (MTB) in cases of smear-negative and clinical diagnosed pulmonary TB. A prospective case-control study was conducted on 473 pulmonary samples received at the tertiary care center from January 2019 to December 2019. All specimens were processed for microscopy and culture. CBNAAT- Gene Xpert and LPA Genotype MTBDRplus (VER 2.0) was used to confirm in smear-negative samples.  Among the pulmonary samples, 52% smear-positive, and 48% smear-negative, 183 (43%) were found to be culture-positive by Lowenstein Jensen medium (LJ) and MGIT 960, 267 (63%) were positive CBNAAT and LPA n= 216 (51%) samples positive for the TUB band. The use of  CBNAAT-Gene Xpert, Line Probe Assay Genotype MTBDR plus(VER 2.0) can be done from the samples directly and the diagnostic performance are more specific for detecting MTB in smear-negative specimens. This study suggests that LPA also helps in the diagnosis of MDR rapidly and in initiation of earlier treatment.

Keywords

Smear-negative Samples, Line probe assay MTBDRplus, CBNAAT, Gene Xpert, MDR, Wild Type and Mutant

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