ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
V. Rajeswari1 , Bhuvaneshwari Gunasekar2 and Selvakkumar Chinnasamy1
1Department of Microbiology, Mahe Institute of Dental Sciences and Hospital, U.T. of Pondicherry, Mahe – 673 312, India.
2Department of Microbiology, Saveetha Medical College, Saveetha University, Chennai – 600 077, Tamilnadu, India.
Article Number: 7867 | © The Author(s). 2022
J Pure Appl Microbiol. 2022;16(3):1931-1942.
Received: 26 May 2022 | Accepted: 18 July 2022 | Published online: 12 August 2022
Issue online: September 2022

Bacteria control gene expression by quorum sensing (QS) mechanism owing to producing small signal molecules associated with population density. Both gram-positive and gram-negative bacteria use QS to manage various physiological characteristics, including bioluminescence, virulence gene expression, biofilm formation, and antibiotic resistance. Impatience balsamina is a flowering, perennial and annual herb indigenous to southern Asia in India. All parts of Impatience balsamina have a therapeutic effect on different diseases. This study evaluated the anti-quorum sensing activity of leaf extract of Impatience balsamina by examining its action on Violacein production by Chromobacterium violaceum, a biosensor strain, and Biofilm, Pyocyanin, Protease, and Chitinase production by the reference strain Pseudomonas aeruginosa PA 01. Minimum inhibitory concentration (MIC) for Pseudomonas aeruginosa PA 01was 3.125mg/ml. A concentration of 1.563mg/ml (sub-MIC) showed inhibition of 100% on Las A protease, 78.42% on chitinase, 30.75% on biofilm, and 93.33% on pyocyanin production by Pseudomonas aeruginosa PA 01. This article displayed the quorum quenching activity of Impatience balsamina by hindering the quorum-sensing controlled characteristics of bacteria without killing it, which reduces the proneness of drug resistance in bacteria, a globally accepted emerging problem in the medical field.


Impatience balsamina, Quorum Sensing, Pyocyanin, Biofilm

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© The Author(s) 2022. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.