ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
V. Rajeswari1 , Bhuvaneshwari Gunasekar2 and Selvakkumar Chinnasamy1
1Department of Microbiology, Mahe Institute of Dental Sciences and Hospital, U.T. of Pondicherry, Mahe – 673 312, India.
2Department of Microbiology, Saveetha Medical College, Saveetha University, Chennai – 600 077, Tamilnadu, India.
Article Number: 7867 | © The Author(s). 2022
J Pure Appl Microbiol. 2022;16(3):1931-1942. https://doi.org/10.22207/JPAM.16.3.40
Received: 26 May 2022 | Accepted: 18 July 2022 | Published online: 12 August 2022
Issue online: September 2022
Abstract

Bacteria control gene expression by quorum sensing (QS) mechanism owing to producing small signal molecules associated with population density. Both gram-positive and gram-negative bacteria use QS to manage various physiological characteristics, including bioluminescence, virulence gene expression, biofilm formation, and antibiotic resistance. Impatience balsamina is a flowering, perennial and annual herb indigenous to southern Asia in India. All parts of Impatience balsamina have a therapeutic effect on different diseases. This study evaluated the anti-quorum sensing activity of leaf extract of Impatience balsamina by examining its action on Violacein production by Chromobacterium violaceum, a biosensor strain, and Biofilm, Pyocyanin, Protease, and Chitinase production by the reference strain Pseudomonas aeruginosa PA 01. Minimum inhibitory concentration (MIC) for Pseudomonas aeruginosa PA 01was 3.125mg/ml. A concentration of 1.563mg/ml (sub-MIC) showed inhibition of 100% on Las A protease, 78.42% on chitinase, 30.75% on biofilm, and 93.33% on pyocyanin production by Pseudomonas aeruginosa PA 01. This article displayed the quorum quenching activity of Impatience balsamina by hindering the quorum-sensing controlled characteristics of bacteria without killing it, which reduces the proneness of drug resistance in bacteria, a globally accepted emerging problem in the medical field.

Keywords

Impatience balsamina, Quorum Sensing, Pyocyanin, Biofilm

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