Bacteria control gene expression by quorum sensing (QS) mechanism owing to producing small signal molecules associated with population density. Both gram-positive and gram-negative bacteria use QS to manage various physiological characteristics, including bioluminescence, virulence gene expression, biofilm formation, and antibiotic resistance. Impatience balsamina is a flowering, perennial and annual herb indigenous to southern Asia in India. All parts of Impatience balsamina have a therapeutic effect on different diseases. This study evaluated the anti-quorum sensing activity of leaf extract of Impatience balsamina by examining its action on Violacein production by Chromobacterium violaceum, a biosensor strain, and Biofilm, Pyocyanin, Protease, and Chitinase production by the reference strain Pseudomonas aeruginosa PA 01. Minimum inhibitory concentration (MIC) for Pseudomonas aeruginosa PA 01was 3.125mg/ml. A concentration of 1.563mg/ml (sub-MIC) showed inhibition of 100% on Las A protease, 78.42% on chitinase, 30.75% on biofilm, and 93.33% on pyocyanin production by Pseudomonas aeruginosa PA 01. This article displayed the quorum quenching activity of Impatience balsamina by hindering the quorum-sensing controlled characteristics of bacteria without killing it, which reduces the proneness of drug resistance in bacteria, a globally accepted emerging problem in the medical field.