ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Miriam Toxqui-Munguia1,5, Raul Avila-Sosa3,5, Elsa Castaneda-Roldan1,5, Esperanza Duarte-Escalante4, Dolores Castaneda-Antonio1, Gloria Leon-Tello2 and Ricardo Munguia-Perez1,5
1Mycology Laboratory, Center for Research in Microbiological Sciences, Institute of Sciences, Benemerita Universidad Autonoma de Puebla, Puebla, Mexico.
2Department of Microbiology, Faculty of Chemical Sciences, Benemerita Universidad Autonoma de Puebla, Puebla, Mexico.
3Department of Biochemistry-Food, Faculty of Chemical Sciences, Benemerita Universidad Autonoma de Puebla, Puebla, Mexico.
4Department of Microbiology and Parasitology, School of Medicine, Universidad Nacional Autonoma de Mexico.
5Postgraduate in Environmental Sciences, Institute of Sciences, Benemerita Universidad Autonoma de Puebla, Puebla, Mexico.
Article Number: 7643 | © The Author(s). 2022
J Pure Appl Microbiol. 2022;16(3):1878-1883. https://doi.org/10.22207/JPAM.16.3.34
Received: 26 February 2022 | Accepted: 05 July 2022 | Published online: 12 August 2022
Issue online: September 2022
Abstract

This study was designed to identify the bacteria and yeasts from the milk samples of dairy cattle presenting with subclinical mastitis and evaluate their antimicrobial susceptibility. We collected a total of 52 milk samples from cows across three farms in San Salvador El Seco (Puebla, Mexico). Microbial isolation was performed using microbiological techniques followed by taxonomic identification of bacteria and yeasts. Antimicrobial susceptibility was evaluated using the guidelines provided by the Clinical Laboratory Standard Institute (CLSI). 1 We identified three genera and six species of yeasts including Candida glabrata, C. krusei, C. lipolytica, Cryptococcus laurentii, Rhodotorula rubra, and R. glutinis and five species of bacteria, including Staphylococcus saprophyticus, S. aureus, S. hominis, S. epidermidis, and Streptococcus disgalactiae. All of the yeast strains were sensitive to amphotericin B; 1/23 (4.3%) were resistant to ketoconazole and nystatin, 10/23 (43%) were resistant to fluconazole, and 13/23 (53%) were resistant to 5-fluorocytosine. The dominant genus isolated was Candida, with the most abundant groups being C glabrata and C. krusei. Resistance to 5-fluorocytosine was observed in all yeasts except C. lipolytica, while both S. aureus and S. epidermidis were resistant to oxacillin and dicloxacillin. S. hominis was resistant to gentamicin. These antimicrobials are still used in bovine therapy for mastitis, directly affecting healthy cattle and, therefore, raw milk.

Keywords

Antimicrobial, Bacteria, Yeasts, Mastitis

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