ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Fatima Moeen Abbas
Department of Biology, College of Sciences for Women, University of Babylon, Iraq.
Article Number: 7667 | © The Author(s). 2022
J Pure Appl Microbiol. 2022;16(3):1873-1877. https://doi.org/10.22207/JPAM.16.3.33
Received: 08 March 2022 | Accepted: 28 June 2022 | Published online: 03 August 2022
Issue online: September 2022
Abstract

The current work suggested the occurrence of blaNDM-1 gene among Klebsiella pneumoniae recovered from surface waters of the Al-Hillah River. Between January and April 2015, water samples (101) were taken from seven different area of the Al-Hillah River, Babylon province, Iraq. K.pneumoniae was reported in percentage of 35 (34.6%). The antibiotics susceptibility profile of K.pneumoniae was determined with disk diffusion assay. The most common resistance was detected for penicillins agents (ampicillin and cloxacillin) with 20(57.14%) and 17(48.57%) resistance rate, respectively. Two isolates of K.pneumoniae were carbapenem-resistant. Phenotypic screening of metallo β-lactamase detection was carried out using imipenem–EDTA double disk synergy test for carbapenem resistant isolates, 2(100%) isolates with positive result. Conventional Polymerase Chain Reaction (PCR) test was used for detection NDM-1 beta-lactamase, 1 (50%) K.pneumoniae isolate harboring this gene.

Keywords

Carbapenem Resistance, Klebsiella pneumoniae, blaNDM-1 Gene, PCR, River Water

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