ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Manaji Pawar and Geeta Karande
Department of Microbiology, Krishna Institute of Medical Sciences, KIMS (Deemed to Be University), Karad (Satara) – 415 539, Maharashtra, India.
Article Number: 7640 | © The Author(s). 2022
J Pure Appl Microbiol. 2022;16(3):1855-1860. https://doi.org/10.22207/JPAM.16.3.31
Received: 25 February 2022 | Accepted: 24 June 2022 | Published online: 03 August 2022
Issue online: September 2022
Abstract

Acute diarrheal illness is a serious public health issue in developing and developed economies, with high morbidity and mortality rates. Children below five years are the most vulnerable, with one in every six dying from diarrhea. Diarrhea may be caused by various viruses, protozoa, and bacteria. Rotavirus, in particular, is often associated with diarrhea in children. Early detection of rotavirus infection reduces the need for medication and the spread of the virus and helps determine the optimal treatment strategies. In this study, we compared the efficacy of the rapid immunochromatography technique (ICT) with that of enzyme-linked immunosorbent assay (ELISA) to detect rotavirus antigens in stool samples. Overall, 361 stool samples were obtained from hospitalized children under five-years-old, with acute watery diarrhea symptoms and were tested by both rapid ICT and ELISA. Among these samples, 104 (28.81%) tested positive by ICT and 109 (30.19%) by ELISA. Furthermore, the specificity and sensitivity of ICT were assessed using ELISA, the gold standard test. ICT had a 95.41% sensitivity and a 100% specificity. Thus, during emergency testing, ICT may be utilized as an alternative test for quick diagnosis with high specificity and sensitivity in patients with rotavirus diarrhea.

Keywords

Acute diarrhea, Rotavirus, Immunochromatography, ELISA, Sensitivity, Specificity

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© The Author(s) 2022. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.