ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
C. Priscilla1, Santosh Kumar1, C.P. Girish Kumar2, Sreejith Parameswaran3, Pragasam Viswanathan4 and Rajesh Nachiappa Ganesh1
1Department of Pathology, Jawaharlal Institute of Postgraduate Medical Education and Research (JIPMER), Puducherry – 605 006, India.
2Laboratory division, National Institute of Epidemiology, Indian Council of Medical Research, Chennai – 600 077, Tamil Nadu, India.
3Department of Nephrology, Jawaharlal Institute of Postgraduate Medical Education and Research (JIPMER), Puducherry – 605 006, India.
4Renal Research Lab, Biomedical Research Centre, School of Biosciences and Technology, VIT University, Vellore – 632 014, Tamil Nadu, India.
Article Number: 7503 | © The Author(s). 2022
J Pure Appl Microbiol. 2022;16(3):1799-1808. https://doi.org/10.22207/JPAM.16.3.23
Received: 22 December 2021 | Accepted: 28 June 2022 | Published online: 03 August 2022
Issue online: September 2022
Abstract

The advent of technologies has made allogenic transplantation a potential curative therapy for end-stage renal diseases, but the episodes of rejection still remain as one of the challenges in the post-transplant scenario. In the recent years, several human and animal studies have elucidated that gut microbial dysbiosis is closely linked with allogenic transplantation and post-transplant complications. But most of the studies focused on the use of high through-put sequencing technologies to analyze gut microbiota despite of its high cost, analysis and time constraints. Hence, in this work we aimed to study the impact of the two dominant gut phyla Firmicutes and Bacteroidetes on 38 renal transplant recipients, before and after transplantation and to find its association with allograft rejection. Significant changes (p<0.01) were observed in the relative abundances of the phyla Firmicutes and Bacteroidetes at pre- and post-transplant period. We have also found that the recipients who had an increase in Firmicutes/Bacteroidetes (F/B) ratio before transplant were highly prone to rejection in the first-year post-transplant. The Receiver Operating Characteristic (ROC) curve analysis has shown that the ratio of F/B were able to discriminate between rejection and non-rejection cases with an Area under the ROC Curve (AUC) of 0.91. Additionally, we observed that the ratio of F/B have reduced during the time of rejection postulating that gut microbial dysbiosis has more association with rejection. Thus, the assessment of F/B ratio using qPCR would be of a more practical approach for diagnosis and monitoring of graft function in a cost-effective and timely manner.

Keywords

Gut Microbiota, Firmicutes, Bacteroidetes, Renal Transplantation, Renal Allograft Rejection, qPCR

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