ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Sawsan M. Jabbar AL-Hasnawi1 and Dhiaa H. Jawad Al-khayat2
1Department of Medical Microbiology, College of Medicine, University of Kerbala, Karbala, Iraq.
2Department of Anaesthesia and Intensive Care, Imam Al-Hussein Teaching Medical City, Karbala, Iraq.
Article Number: 7745 | © The Author(s). 2022
J Pure Appl Microbiol. 2022;16(3):1792-1798. https://doi.org/10.22207/JPAM.16.3.22
Received: 07 April 2022 | Accepted: 04 July 2022 | Published online: 03 August 2022
Issue online: September 2022
Abstract

The heterogeneous etiology of asthma makes its diagnosis complicated. Measurement of cytokine levels could be relevant in determining the asthma phenotype, predicting severity, and identifying the treatment type. Enzyme-linked immunosorbent assay (ELISA) is one of the most reliable methods, with high sensitivity and specificity. This study aimed to determine the accuracy and utility of interleukin (IL)-13 and IL-17 A in diagnosing children with asthma. A total of 74 asthmatic and 75 healthy children were enrolled in this case-control study between 10/2019 and 3/2021. Sera were collected and analyzed for IL-13 and IL-17A using ELISA. Diagnostic utility assessment was performed using receiver operating characteristic (ROC) analysis. The results showed that both cytokines had a significant capacity to differentiate patients with asthma from the control group. The sensitivity and specificity for IL-17A were 97.3% and 52.0%, respectively, whereas for IL-13 it was 81.1% and 52.0%, respectively. Positive predictive values (PPV) were 66.7% and 62.5% for IL-17A and IL-13, respectively. In conclusion, although both biomarkers had low specificity, IL-17A was more sensitive in differentiating children with asthma from those in the control group and had a higher sensitivity rate than IL-13.

Keywords

IL-13, IL-17A, ELISA, Diagnostic Utility, Asthma

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© The Author(s) 2022. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.