ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Wen-Yang Lin , Yi-Wei Kuo, Ching-Wei Chen, Yu-Chieh Hsu, Yu-Fen Huang, Chen-Hung Hsu, Jia-Hung Lin, Chi-Huei Lin, Cheng-Chi Lin, Tsai-Hsuan Yi, Yu-Wen Chu and Hsieh-Hsun Ho
Department of Research and Design, Glac Biotech Co., Ltd., Tainan, Taiwan.
Article Number: 7666 | © The Author(s). 2022
J Pure Appl Microbiol. 2022;16(3):1771-1782.
Received: 08 March 2022 | Accepted: 23 May 2022 | Published online: 25 July 2022
Issue online: September 2022

Dysbiosis of intestinal microbiota may cause irregular digestive function, and intestinal wall inflammation. Over the past few years, probiotics generate bioactive metabolites, named postbiotics, have been discovered its crucial roles in modulation of intestinal microbiota. Single-strain postbiotics have positive effect on health of host, but the functions of multi-strain postbiotics remain unclear. This study proposed a useful application of multi-strain postbiotics and thereby establish the developing foundation of multi-strain postbiotics. Initially, various probiotics and postbiotics were screened for anti-inflammatory activity through inducing the transforming growth factor beta (TGF-β)and interleukin-10 (IL-10) in peripheral blood mononuclear cells (PBMCs). Then, we detailed the synergistic effects of 4Mix postbiotics (named as Probiotic Extracts of 4 strains- number 1, PE0401) consisted of metabolites generated from Lactobacillus salivarius AP-32, Lactobacillus acidophilus TYCA06, Lactobacillus plantarum LPL28, Bifidobacterium longum subsp. infantis BLI-02 on anti-inflammatory activity, anti-oxidative capacity, regulation of tight junction proteins. The results displayed that anti-inflammatory activity of 4Mix postbiotics PE0401 was stronger than other mixed postbiotic combinations. The anti-oxidative capacity, which correlated to anti-inflammation, also significantly increased as shown in DPPH and FRAP assays. The epithelial tight junction proteins expressed in mRNA levels (ZO-1, ZO-2, Occludin, JAM-A, and Claudin) were highly potent modulated by PE0401. In addition, PE0401 selectively promoted the growth of intestinal bacterial strains including Lactobacillus, Bifidobacterium strains and other beneficial bacteria. Therefore, this study provides a fascinating insight into the strategy to the treatment of the intestinal disorders. PE0401 may deliver as health functional food ingredient.


Postbiotic, Chronic Inflammatory Bowel Disease (IBD), Anti-inflammatory, Anti-oxidation, Tight Junction Protein, Growth Promotion of Beneficial Bacteria

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© The Author(s) 2022. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.