Healthcare professionals, especially those who work in hospitals that treat patients with Coronavirus Infection (COVID-19), are at a higher risk of contracting this disease than workers in other departments. Healthcare workers are under a lot of stress as they fight the COVID-19 outbreak, which increases their risk of developing psychological difficulties. As a result, ill-prepared healthcare workers can lead to delayed diagnosis, disease spread, and poor infection control. An evaluation of Knowledge, Attitudes, Perceptions, and Psychological Effects in light of the COVID-19 pandemic in Libya may indicate effective strategies for behavioral change in a given society and may assess the psychological state of healthcare workers during that crisis. At present, Libyan healthcare workers are not well aware of the risks. Healthcare workers participated in a web-based cross-sectional survey during the COVID-19 pandemic in 2020. The KAP questions are imported from Egyptian study and the psychological impact questions are imported from Chinese study that used the Generalized Anxiety Disorder Scale, Patient Health Questionnaire and Insomnia Severity Index. Questions are adapted to be suitable for the Libyan context. The Knowledge, attitude and perception were analyzed using a total score based on Bloom’s 80% cut-off. According to our results, in total, we received 85.4% high knowledge score of Libyan HCWs in regards to COVID-19. About 54.6% participants showed negative attitude towards COVID-19 infection and the precautionary measures taken to prevent its spread. The majority of respondents, 89.1%, felt that they were vulnerable to COVID-19 infection. 85.4% believed they were more likely than others to become infected. Fear of transmission to family members, having chronic illnesses, and community stigma are the most frequently reported reasons for higher risk perceptions. In general, HCWs had a good understanding of COVID-19. Negative attitudes are evident in the observations a high level of risk perception was evident. It is vital that the Libyan Ministry of Health and government consider the reasons for increased risk perception. Healthcare workers, specifically those who are responding to COVID-19 in Libya, experienced an increase in anxiety, depression and insomnia, which were respectively 30.1%, 31.9% and 18.9%. In this study, many Healthcare workers reported adequate overall knowledge with a negative attitude toward government and adopted appropriate practices. Psychological problems are directly related to inadequate knowledge, incorrect attitudes, and inadequate perception. However, with timely interventions and correct information, it will be possible to protect the mental wellbeing of healthcare professionals during the novel coronavirus epidemic.