ISSN: 0973-7510

E-ISSN: 2581-690X

Review Article | Open Access
Srivalsa Bhaskaran and Chithralekha Saikumar
Department of Microbiology, Sree Balaji Medical College and Hospital, Chromepet, Chennai – 600 044, Tamil Nadu, India.
J Pure Appl Microbiol. 2022;16(2):825-833 | Article Number: 7412 | © The Author(s). 2022
Received: 06/11/2021 | Accepted: 14/05/2022 | Published online: 31/05/2022
Issue online: June 2022

Next-generation sequencing (NGS) is a new technology used to detect the sequence of DNA and RNA and to detect mutations or variations of significance. NGS generates large quantities of sequence data within a short time duration. The various types of sequencing includes Sanger Sequencing, Pyrosequencing, Sequencing by Synthesis (Illumina), Ligation (SoLID), Single molecule Fluorescent Sequencing (Helicos), Single molecule Real time Sequencing (Pacbio), Semiconductor sequencing (Ion torrent technology), Nanopore sequencing and fourth generation sequencing. These methods of sequencing have been modified and improved over the years such that it has become cost effective and accessible to diagnostic laboratories. Management of Outbreaks, rapid identification of bacteria, molecular case finding, taxonomy, detection of the zoonotic agents and guiding prevention strategies in HIV outbreaks are just a few of the many applications of Next Generation sequencing in clinical microbiology.


Next generation sequencing, Sangers, Illumina, SoLiD, Outbreak management

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