ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Vimolmas Tansathitaya1 , Witchana Sarasin2, Tanapati Phakham2,Vorthon Sawaswong3, Prangwalai Chanchaem3 and Sunchai Payungporn3
1College of Sports Science and Technology, Mahidol University, Nakhon Pathom 73170, Thailand.
2Center of Excellence in Systems Biology, Faculty of Medicine, Chulalongkorn University,
Bangkok 10330, Thailand.
3Research Unit of Systems Microbiology, Department of Biochemistry, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand.
J Pure Appl Microbiol. 2022;16(2):1130-1137 | Article Number: 7545
https://doi.org/10.22207/JPAM.16.2.39 | © The Author(s). 2022
Received: 14/01/2022 | Accepted: 01/04/2022 | Published online: 27/05/2022
Issue online: June 2022
Abstract

The microbiome, a community of microorganisms in the body, is currently used as a biomarker in many disease prognoses. Prevotella, Turicibacte, Bacteroides, Firmicutes/Bacteroidetes are frequently used as a biomarker for rheumatoid arthritis, colorectal cancer, and obesity in ordered. The amount of gut microbiota can be changed depending on various factors such as diet, lifestyle, and exercise. However, there is unclear on how the exercise is really effective to be a disease prevention. The present study aims to investigate the different exercise intensities on gut microbiome abundance changes that could be used as a disease biomarker. Eighteen Sprague-Dawley rats were arranged (n=6 per group) into 3 exercise intensity levels on treadmills including non-exercise group, high -exercise group (20 – 25 m/min for 60 min), and light-exercise group (10 – 15 m/min for 60 min). Rats were weighted every 2 days and stools were collected and preserved in DNA/RNA shield each week. The bacterial 16S rDNA of microbiome in feces samples was sequenced and analyzed. After week eighth of the interventions, from operational taxonomic unit (OTUs) abundance, we found that the relative abundance in bacterial genera in Prevotella and Firmicutes/Bacteroidetes were significantly correlated with the experiment timepoints in different exercise intensities (Pearson’s correlation, P<0.05) compare to other genera. The exercise intensities and exercise durations can affect the relative abundance in the bacteria genus which the abundance genus Prevotella and Firmicutes/Bacteroidetes could be used as a new standard biomarker in exercise as a disease prevention and exercise prescriptions. From the funding limitations, we could conclude the research results based on our data and statistic. Future research should utilize a longer investigation period.

Keywords

Aerobic exercise, Firmicutes/Bacteroidetes, Prevotella, Turicibacter

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© The Author(s) 2022. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.