ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Shanmugapriya Thiagarajan1, Selvaraj Stephen2, Santosh Kumar3,Priscilla Charles3, Sarangapani Kanagamuthu1, Stanley Ambroise4, Pragasam Viswanathan5, Palanivel Chinnakali6 and Rajesh Nachiappa Ganesh3
1Department of Microbiology, Indira Gandhi Government General Hospital and Postgraduate Institute, Puducherry – 605 001, India.
2Department of Microbiology, Mahatma Gandhi Medical College and Research Institute, Sri Balaji Vidyapeeth Deemed to be University Puducherry – 607 403, India.
3Department of Pathology, Jawaharlal Institute of Postgraduate Medical Education and Research, Puducherry – 605 006, India.
4Department of Medicine, Indira Gandhi Government General Hospital and Postgraduate Institute, Puducherry – 605 001, India.
5School of Biological Sciences and Technology, Vellore Institute of Technology, Vellore – 632 014, Tamil Nadu, India.
6Department of Preventive and Social Medicine, Jawaharlal Institute of Postgraduate Medical Education and Research, Puducherry – 605006, India.
J Pure Appl Microbiol. 2022;16(2):1074-1083 | Article Number: 7559
https://doi.org/10.22207/JPAM.16.2.32 | © The Author(s). 2022
Received: 21/01/2022 | Accepted: 29/03/2022 | Published online: 17/05/2022
Issue online: June 2022
Abstract

Acute kidney injury (AKI) is a leading cause of morbidity in urinary tract infection (UTI). We aimed to identify simple biomarkers and bacterial virulence factors associated with AKI in a setting of upper UTI due to uropathogenic Escherichia coli (UPEC). We designed a cross-sectional study to analyse biomarkers of AKI in upper UTI patients. A total of 2758 patients presenting to a tertiary care center with symptoms of upper UTI were assessed for the presence of diabetes mellitus, AKI, blood group non-secretors, urine microscopy and hemogram. 300 patients with UPEC in bacterial culture were studied for bacterial virulence factors by phenotypic and genotypic methods and the patients were followed up for a minimum period of two weeks. Patients with diabetes mellitus, non-secretors, Urine microscopy (UM) score > 2 and higher (> 3.9) neutrophil-lymphocyte ratio (NLR) at initial presentation, were found to be significantly associated with AKI at presentation and predicted AKI at 7th and 14th day follow up. They were also significantly associated with need and increased duration of hospitalization. There was no association of virulence factors of UPEC with diabetes mellitus, AKI or need for hospitalization. However, virulence factors had a significant association (p<0.001)with non-secretors. UM score and NLR are simple tools to identify AKI at presentation and predict AKI during follow-up and the need for hospitalization. Patients with diabetes mellitus and non-secretors are also at higher risk of AKI. Non-secretors are significantly associated with both phenotypic and genotypic expression of virulence factors of UPEC.

Keywords

Acute Kidney Injury, Neutrophil lymphocyte ratio, Urine microscopy score, non-secretors, Virulence factors of Uropathogenic Escherichia coli

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