ISSN: 0973-7510

E-ISSN: 2581-690X

Review Article | Open Access

Mohammad Irfan1 and Dakshina Bisht2

1Department of Clinical Laboratory Science, College of Applied Medical Science-Ad Dawadmi, Shaqra University, 17464 Ad Dawadmi, Kingdom of Saudi Arabia.
2Department of Microbiology, Santosh Medical College, Santosh University, Ghaziabad – 201 009, Uttar Pradesh, India.
J Pure Appl Microbiol. 2022;16(1):74-88 | Article Number: 7491 | © The Author(s). 2022
Received: 14/12/2021 | Accepted: 02/02/2022 | Published online: 01/03/2022
Issue online: March 2022

Tuberculosis (TB) continues to be a significant public health concern on a global scale. Quick and precise identification of Mycobacterium tuberculosis (MTB) in symptomatic patients is pivotal for worldwide TB eradication initiatives. As an infectious disorder induced by MTB, it remains a critical threat to public health, particularly in poor countries, due to an inadequate diagnostic research laboratory. There is a need for a persistent incentive to reduce response time for effective diagnosis and control of TB infection, which is a benefit that molecular techniques provide over traditional methods. Although there is a tremendous overall prevalence of TB and a relatively poor probability of case identification worldwide. Common screening techniques have focused on tests that have many fundamental shortcomings. Due to the development of antibiotic-resistant Mycobacterium strains, TB is one of the leading contributors to fatalities. It is now possible to examine TB using molecular detection techniques, which are faster and more cost-effective than previous methods, such as standard culture procedures to test and verify antibiotic resistance in patients with TB. Whole genome sequencing (WGS), faster nucleic acid amplification tests, has made it easier to diagnose and treat TB more quickly. This article addresses the genetic approaches for detecting Mycobacterium tuberculosis complex (MTBC) in clinical specimens as well as antibiotic resistance in mycobacterium and discusses the practical limitations of using these methods.


Nucleic acid amplification test, tuberculosis, Mycobacterium tuberculosis complex (MTBC), diagnosis of tuberculosis, Molecular Identification

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© The Author(s) 2022. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.