Purpose of the study: To estimate the prevalence of HIV 1 and HIV 2 infection in patients attending the tertiary care setup. Routine screening for HIV infection of 10000 samples were carried out between July 2017-June 2018. All patients were given a pre-test counselling and filled up the Consent form provided by NACO. The samples were subjected to the NACO provided Comb Aids RS Advantage kit and all positives were tested using 2 other kits Meriscreen HIV 1-2 and Tridot Test for differentiation. The data thus obtained were charted in Excel and the prevalence were determined. A Post-test counselling was given for all patients and the results were disclosed following the protocol provided by NACO. Of the 10000 samples, 82 (0.82%) samples were reactive for HIV. In the 82 reactive samples, 78 (95.12%) samples were reactive for HIV- 1 antibodies and 4 (4.8%) samples were reactive for HIV-2 antibodies. Among the 82 reactive clients, 53 clients were male and 29 clients were female.