ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Huda Mohammed Ahmed Sheikh1,2 , Ola I.M. Hamshary3, Abd El-Nasser Abd El-Hafez Khattab4
1Department of Biology, Faculty of Science, University of Jeddah, Jeddah 21589, Saudi Arabia.
2Department of Biological Science, Science and Arts College, Rabigh Campus, King Abdulaziz University, Jeddah, Saudi Arabia.
3Department of Microbial Genetics, National Research Centre, Dokki, Cairo-12622, Egypt.
4Department of Genetics and Cytology, National Research Centre, Dokki, Cairo-12622, Egypt.
J Pure Appl Microbiol. 2022;16(1):643-654 | Article Number: 7225
https://doi.org/10.22207/JPAM.16.1.66 | © The Author(s). 2022
Received: 06/08/2021 | Accepted: 25/01/2022 | Published online: 25/02/2022
Issue online: March 2022
Abstract

Bacillus bacteria are advantageous antagonistic organisms that can be used as bio-control agents. This study is aimed at screening the antagonistic activity of different strains of isolated Bacillus bacteria and molecular identification of the superior chitinase producer strain against dermatophytes fungi. Soil samples were collected from different places of Kotoor city, Gharbia Governorate, Egypt and Al Madina Al Munawwarah, Kingdom of Saudi Arabia. A collection of Bacillus isolated from soil was tested in vitro against the dermatophytes: Microsporum sp. and Trichophyton sp. The bacterial strains Kh-B1 and Kh-B2 showed the highest antagonistic activity against dermatophytes pathogenic fungi. The highest amount of chitinase productivity (13.6 units/ml) was obtained from the original Bacillus strain (Kh-B1) at 3 days of incubation. BLAST analysis of the amplified 16S ribosomal RNA gene sequence identified the Bacillus strain (Kh-B1) as Paenibacillus macerans. Upon the mutation induction by UV light, the highest chitinase-producing mutant was Kh-UVB-4 as it showed 305.88 percent production higher than the wild-type strain. While, upon the mutation induction by EMS, the highest amount of chitinase produced was 54.8 units/ml by mutant Kh-ESB-20, and it has produced 402.94% more than the original untreated strain. The application of RAPD-PCR protocol using three 15-mer random primers was used to determine the genetic effects of mutagenic treatments on the wild type strain (Kh-B1) as well as to demonstrate the genetic variability between the five most chitinase producing mutants and the wild type (Paenibacillus macerans).

Keywords

Bacillus strain, Chitinase producer, UV and EMS mutagens, antagonistic activity, RAPD-PCR

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