ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Mohammed A. Almalki1, Ashraf Y.Z. Khalifa1,2 and Yousef A. Alkhamis3,4
1Department of Biological Sciences, College of Science, King Faisal University, Al-Ahsa, Saudi Arabia.
2Department of Botany and Microbiology, Faculty of Science, Beni-Suef University, Beni-Suef, Egypt.
3Department of Animal and Fish Production, College of Agriculture Science & Food Science, King Faisal University, Al-Ahsa, Saudi Arabia.
4Fish Resources Research Center, King Faisal University, Al-Ahsa, Saudi Arabia.
J Pure Appl Microbiol. 2022;16(1):630-637 | Article Number: 7505
https://doi.org/10.22207/JPAM.16.1.64 | © The Author(s). 2022
Received: 20/12/2021 | Accepted: 12/01/2022 | Published online: 25/02/2022
Issue online: March 2022
Abstract

The aim of the current study was to evaluate the antibacterial activity of Chlorella vulgaris extracts against Stenotrophomonas maltophilia CSK1 isolated from a diseased cucumber fruit. To this end, water, methanol and ethanol were used separately as solvents to extract the active compounds from the dried Chlorella vulgaris. S. maltophilia CSK1 was identified using the 16S rRNA gene sequencing at 99.93% of identity level. The identity of the bacterial strain CSK1 was confirmed by phylogenetic analyses. CSK1 was clearly clustered with the reference strain, S. maltophilia MTCC 434 (JALV01000036). The antibiosis of the extract against S. maltophilia CSK1 was estimated using agar well diffusion method. Unlike water and ethanolic extracts, the methanolic one exhibited an inhibition of CSK1 (zone of inhibition 13.4 mm), indicating the antimicrobial activity of this extract. No zone of inhibition was formed around ethanolic and water extracts, indicating that both exhibited no-observed effect against the growth of CSK1. This can be attributed to the active constituents in the extract which may varies based on various factors including the polarity of solvent used in the extraction process. The inhibitory effect of the methanolic extract against the S. maltophilia CSK1 obtained from the diseased cucumber fruit could open the door for potential application of such active ingredient within this extract against this bacteria in future.

Keywords

Chlorella vulgaris, Stenotrophomonas maltophilia, antimicrobial activity

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