ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Silla Varghese Thomas and K. Gopalakrishna Bhat
Department of Microbiology, Kasturba Medical College, Manipal Academy of Higher Education, Manipal, Mangalore – 575 001, Karnataka, India.
J Pure Appl Microbiol. 2022;16(1):613-619 | Article Number: 7213 | © The Author(s). 2022
Received: 02/08/2021 | Accepted: 28/01/2022 | Published online: 25/02/2022
Issue online: March 2022

Healthcare-Associated Infections (HAIs) are of global concern in this present era and white coats play an important role in the transmission of HAIs. The most common healthcare-associated bacteria are Enterococcus species, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumanni, Pseudomonas aeruginosa and Enterobacter species (ESKAPE bugs). These healthcare-associated bacteria are capable of surviving on white coats which could act as fomites in the transmission of HAIs. The purpose of this study was to compare the duration of survival of healthcare-associated bacteria on different materials used for making white coats. Materials like pure cotton, artificial cotton, cotton silk, spun and crepe were cut into swatches of 1cm2 size and sterilized by autoclaving. Five different bacteria isolated from clinical specimens were grown on 5 % sheep blood agar and bacterial suspensions were made in sterile physiological saline. The swatches were immersed in bacterial suspension and kept in petri plates at 25°C. The viable counts of bacteria were determined at definite time intervals by surface plate method. The present study shows that among the healthcare-associated bacteria, S.aureus survived the maximum up to 52 days. The duration of survival of S.aureus was significantly longer than P.aeruginosa and A.baumannii (p <0.05). A.baumannii survived only up to 20 days maximum. All the healthcare-associated bacteria significantly survived for the shortest duration of time on crepe. Therefore, crepe could be a better material used for making white coats.


Healthcare-Associated bacteria, Survival, White coat materials, Fomites, Gram-positive cocci, Gram-negative bacilli

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© The Author(s) 2022. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.