Healthcare-Associated Infections (HAIs) are of global concern in this present era and white coats play an important role in the transmission of HAIs. The most common healthcare-associated bacteria are Enterococcus species, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumanni, Pseudomonas aeruginosa and Enterobacter species (ESKAPE bugs). These healthcare-associated bacteria are capable of surviving on white coats which could act as fomites in the transmission of HAIs. The purpose of this study was to compare the duration of survival of healthcare-associated bacteria on different materials used for making white coats. Materials like pure cotton, artificial cotton, cotton silk, spun and crepe were cut into swatches of 1cm² size and sterilized by autoclaving. Five different bacteria isolated from clinical specimens were grown on 5 % sheep blood agar and bacterial suspensions were made in sterile physiological saline. The swatches were immersed in bacterial suspension and kept in petri plates at 25°C. The viable counts of bacteria were determined at definite time intervals by surface plate method. The present study shows that among the healthcare-associated bacteria, S.aureus survived the maximum up to 52 days. The duration of survival of S.aureus was significantly longer than P.aeruginosa and A.baumannii (p <0.05). A.baumannii survived only up to 20 days maximum. All the healthcare-associated bacteria significantly survived for the shortest duration of time on crepe. Therefore, crepe could be a better material used for making white coats.