ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Shaikh M Shagufta and P.V. Dharani
Department of Microbiology, Sheth P.T. Arts and Science College, Shri Govind Guru University, Godhra – 389 001, Gujarat, India.
J Pure Appl Microbiol. 2022;16(1):567-577 | Article Number: 7291 | © The Author(s). 2022
Received: 05/09/2021 | Accepted: 24/01/2022 | Published online: 25/02/2022
Issue online: March 2022

Biosurfactant are surface active compounds (SAC), amphiphilic in nature consisting of hydrophobic (long chain fatty acids) and hydrophilic domain (amino acid, protein, carbohydrate). Classification of this compound is based on molecular weight, mode of action and physico-chemical structure. Biosurfactant are being utilized in laundry formulation, house hold cleaning products, as antimicrobial agents, in detergents, pesticides, food and pharmaceutical industries. Various type of microorganism has been studied capable of synthesizing wide range biosurfactant by utilizing hydrocarbon compounds as source of carbon and energy. Present study aims to isolate biosurfactant producing bacteria from different waste sources. In this study among twenty-one isolated microbial cultures from different waste samples were screened for biosurfactant production using methods like hemolytic activity, blue gar assay, hydrocarbon tolerance assay, oil spread test, Emulsification index (E24%).


Biosurfactant, Oil Spreading Method, Emulsification index, Modified Drop Collapse, Tolerance against Hydrocarbon, Hemolytic activity, Blue agar plate, Agar well diffusion

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© The Author(s) 2022. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.