ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Ankur Kumar1, Anita Pandey1 , Amresh Kumar Singh2, Archana Dubey1, Asmita Singh2 and Vivek Gaur2
1Department of Microbiology, Subharti Medical College, Swami Vivekanand Subharti University, Meerut – 250 005, Uttar Pradesh, India.
2Baba Raghav Das Medical College, Gorakhpur – 273 013, Uttar Pradesh, India.
J Pure Appl Microbiol. 2022;16(1):471-480 | Article Number: 7405 | © The Author(s). 2022
Received: 03/11/2021 | Accepted: 15/01/2022 | Published online: 21/02/2022
Issue online: March 2022

Rotavirus is a major cause of severe acute gastroenteritis in infants and young children worldwide. It is responsible for 40% of childhood hospitalization. It has been estimated that about 23% of diarrhoeal death occurs due to the rotavirus infection in India. To study the epidemiology of rotavirus infection in children less than 5 years of age at a tertiary care teaching hospital. A total of 240 stool samples were collected from children <5 years of age suffering from acute diarrhea at a tertiary health care referral hospital in Western Uttar Pradesh, India. Rotavirus antigen was detected in stool by Enzyme immunoassay (EIA) which utilizes monoclonal antibodies directed against VP6 antigen. Molecular genotyping was done by nested multiplex PCR.  The rotavirus antigen positivity rate was found to be 14.58% in this study. There was male preponderance and the male: female ratio was 1.5:1. Rotavirus diarrhoea was reported predominantly (42.85 %) in the age group of 13 to 24 months. The most common circulating G/P genotype strain was G9P[4] accounting for 36% of cases. Rotavirus remains a major cause of diarrhoea in children <5 years of age in Uttar Pradesh. However, the positivity rate has decreased after introduction of rotavirus vaccine into the Universal immunization program (UIP) in UP.


Diarrhoeal infection, Enzyme immunoassay (EIA), Rotavirus vaccine, rotavirus antigen

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© The Author(s) 2022. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.