ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Sonam Gaba1,2, Mayur G. Naitam1, Abha Kumari2, Marnix H. Medema3 and Rajeev Kaushik1
1Division of Microbiology, ICAR-Indian Agriculture Research Institute, New Delhi – 110 012, India.
2Amity Institute of Biotechnology, Amity University, Noida, Uttar Pradesh – 201 313, India.
3Bioinformatics Group, Wageningen University, Wageningen – 6708, Netherlands.
J Pure Appl Microbiol. 2022;16(1):460-470 | Article Number: 7436
https://doi.org/10.22207/JPAM.16.1.44 | © The Author(s). 2022
Received: 17/11/2021 | Accepted: 10/01/2022 | Published online: 21/02/2022
Issue online: March 2022
Abstract

Halophilic archaeon Halolamina pelagica CDK2, showcasing plant growth-promoting properties and endurance towards harsh environmental conditions (high salinity, heavy metals, high temperature and UV radiation) was sequenced earlier. Pan-genome of Halolamina genus was created and investigated for strain-specific genes of CDK2, which might confer it with features helping it to withstand high abiotic stress. Pathways and subsystems in CDK2 were compared with other Halolamina strain CGHMS and analysed using KEGG and RAST. A genome-scale metabolic model was reconstructed from the genome of H. pelagica CDK2. Results implicated strain-specific genes like thermostable carboxypeptidase and DNA repair protein MutS which might protect the proteins and DNA from high temperature and UV denaturation respectively. A bifunctional trehalose synthase gene responsible for trehalose biosynthesis was also annotated specifying the need for low salt compatible solute strategy, the probable reason behind the ability of this haloarchaea to survive in a wide range of salt concentrations. A modified shikimate and mevalonate pathways were also identified in CDK2, along with many ABC transporters for metal uptakes like zinc and cobalt through pathway analysis. Probable employment of one multifunctional ABC transporter in place of two for similar metals (Nickel/cobalt and molybdenum/tungsten) might be employed as a strategy for energy conservation. The findings of the present study could be utilized for future research relating metabolic model for flux balance analysis and the genetic repertoire imparting resistance to harsh conditions can be transferred to crops for improving their tolerance to abiotic stresses.

Keywords

Halolamina pelagica, Halophilic Archaea, Genome-scale metabolic model reconstruction, Osmotic stress adaptation

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