ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
S. Ranjini Priya and A. Subhashini
Department of Microbiology, Ethiraj College for Women, Egmore, Chennai – 600 008, Tamil Nadu, India.
J Pure Appl Microbiol. 2022;16(1):453-459 | Article Number: 7395
https://doi.org/10.22207/JPAM.16.1.43 | © The Author(s). 2022
Received: 10/10/2021 | Accepted: 08/01/2022 | Published online: 21/02/2022
Issue online: March 2022
Abstract

L-asparaginase is a therapeutic enzyme that converts L-asparagine into ammonia and L-aspartate. L-asparaginase is used to treat acute lymphoblastic leukaemia. In food manufacturing industries, it is used to inhibit the acrylamide formation. The current investigation has been performed to isolate L-asparaginase producing fungi from different medicinal plants and soil samples, through serial dilution. A total number of 15 fungal isolates were obtained from soil samples and 6 endophytic fungi isolated from medicinal plants. By performing screening of L-asparaginase 67% of positive isolates were obtained from endophytes and soil samples. Optimization of L-asparaginase production was performed for parameters such as pH, temperature, carbon and nitrogen source, and it was found that pH 6, 30˚C, 2 g of glucose, and 1 g of L-arginine is suitable for maximum enzyme production. By performing Sodium dodecyl sulphate polyacrylamide gel electrophoresis the molecular weight of an enzyme was determined to be approximately 11.2 kDa.

Keywords

Czapek Dox’s medium, Endophytic fungi, L-Asparaginase, Nesslerization

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