ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Charanjeev Kaur and Sarbjeet Sharma
Department of Microbiology, Sri Guru Ram Das Institute of Medical Sciences and Research, Amritsar, Punjab, India.
J Pure Appl Microbiol. 2022;16(1):448-452 | Article Number: 7231
https://doi.org/10.22207/JPAM.16.1.42 | © The Author(s). 2022
Received: 08/08/2021 | Accepted: 04/01/2022 | Published online: 21/02/2022
Issue online: March 2022
Abstract

Dengue is a mosquito-borne arboviral disease of grave public health concern worldwide. Early diagnosis and treatment is required to reduce morbidity & mortality from complications caused by secondary dengue infection. According to WHO, the three main diagnostic modalities for the diagnosis of dengue infection are cultivation and identification of viruses, molecular methods, and serology. Whereas virus cultivation is labour intensive and available only in reference laboratories, molecular methods require expensive infrastructure & expertise. Serology on the other hand not only less tedious but is also able to differentiate between primary and secondary dengue. This study was undertaken to evaluate the diagnostic efficacy of rapid immunochromatographic assay in the diagnosis of dengue infection as compared to ELISA. The study was conducted in the serology section of the Microbiology laboratory, Sri Guru Ram Das Institute of Medical Sciences, Amritsar. Blood samples from 429 patients with clinical suspicion of dengue virus infection were received in the lab from August 2020 to December 2020. All samples were subjected to rapid ICT and ELISA to detect NS1 Ag and IgM antibodies. The majority number of cases were observed in the age group of 31 to 40 years while the gender-wise ratio was 1.43:1 showing male preponderance. Out of 429 samples tested, 156 were reactive for either NS1 antigen or IgM antibodies by the ELISA method. Results of rapid ICT for NS1Ag and results of NS1Ag by ELISA were analyzed and compared. A sensitivity of 81.25% was noted and specificity of 100%. IgM detection by rapid ICT in comparison to IgM ELISA shows a sensitivity of 82.14% and specificity of 100%. Rapid ICT kits performed at par with the ELISA. Rapid immunochromatographic assays are important diagnostic tools in the identification of dengue and early treatment of dengue patients is possible, reducing mortality significantly.

Keywords

Dengue, Rapid Immunochromatographic test, ELISA

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