ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Bayan M. Abu-Ghazaleh
Department of Biology and Biotechnology, Faculty of Science, Hashemite University, Zarqa, Jordan.
J Pure Appl Microbiol. 2022;16(1):389-393 | Article Number: 7439
https://doi.org/10.22207/JPAM.16.1.33 | © The Author(s). 2022
Received: 18/11/2021 | Accepted: 04/01/2022 | Published online: 15/02/2022
Issue online: March 2022
Abstract

Coloring agents such as allura red and tartrazine are frequently used in food and pharmaceutical industries. Also, many types of preservatives are added to food and pharmaceutical products to prevent their spoilage. This study examined the effect of pretreatment with nutrient broth containing allura red (AR) or tartrazine (TA) on survival of E. coli and Staphylococcus aureus in media containing preservatives (eg ascorbic acid, citric acid, or sodium chloride). Pretreatment of E. coli for 4 days with AR or TA before exposure for 3 days to nutrient broth containing NaCl (20%) significantly increased its resistance to sodium chloride (20%). Pretreatment of E. coli for 4 days with AR, TA, or nutrient broth alone before exposure for 3 days to nutrient broth containing ascorbic acid (1%) or to nutrient broth containing citric acid (0.5%) killed all cells. For S. aureus, pretreatment with AR or TA before exposure for 3 days to nutrient broth containing citric acid (0.3%) significantly increased its resistance to citric acid (0.3%). Pretreatment of S. aureus for 4 days with AR, TA, or nutrient broth alone before exposure for 3 days to nutrient broth containing citric acid (0.3%) reduced number of cells by 4.37, 2.54, and 6.97 logs, respectively. Pretreatment of S. aureus for 4 days with AR, TA, or nutrient broth alone before exposure for 3 days to nutrient broth containing ascorbic acid (0.5%) reduced number of viable cells by 3, 3.6, and 3.07 logs, respectively. Pretreatment of S. aureus for 4 days with AR, TA, or nutrient broth alone before exposure for 3 days to nutrient broth containing NaCl (20%) increased number of cells by 0.74, 0.7 and 1.1logs, respectively. These results have implications in food and pharmaceutical industries.

Keywords

Allura red, tartrazine, S. aureus, E. coli, preservatives

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© The Author(s) 2022. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.