ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Vinod Kumar Yadav1,2, Neeta Bhagat2 and Sushil K. Sharma1,3
1ICAR-National Bureau of Agriculturally Important Microorganisms (ICAR-NBAIM), Kushmaur, Maunath Bhanjan – 275 103, Uttar Pradesh, India.
2Amity Institute of Biotechnology, Amity University Uttar Pradesh, Noida – 201 313, India.
3ICAR-National Institute of Biotic Stress Management, Baronda, Raipur – 493 225, Chhattisgarh, India.
J Pure Appl Microbiol. 2022;16(1):246-262 | Article Number: 7371
https://doi.org/10.22207/JPAM.16.1.14 | © The Author(s). 2022
Received: 13/10/2021 | Accepted: 09/12/2021 | Published online: 10/01/2022
Issue online: March 2022
Abstract

Drought is one of the most detrimental environmental stressors to plants with the potential to decrease crop yields and affect agricultural sustainability. Native bacteria with beneficial traits enhance plant growth and help avoid and reverse the effects of drought in plants to a greater extent. In the present study, we aimed to ( i ) isolate drought-tolerant Bacillus isolates from the rhizosphere soil of wheat crop grown at different locations in Jaisalmer district, Rajasthan state and (ii) further evaluate their ability to enhance plant growth and induce drought tolerance in wheat ( Var. HD-2967) grown under drought stress conditions. Of more than 100 isolates, two putative Bacillus isolates capable of tolerating 30 % polyethylene glycol-6000 (PEG-6000) [equivalent to -9.80 MPa (Megapascal)] were identified as Bacillus altitudinis DT-89 and Bacillus paramycoides DT-113. These isolates exhibited different plant growth promoting (PGP) attributes such as phosphate solubilization, and production of siderophore, exopolysaccharide, ammonia, indole acetic acid and cytokinin at low osmotic stress of 10% PEG-6000 but shown variable response at higher osmotic stress particularly at 30% PEG-6000. However, they did not show any antifungal activity and one isolate was negative for phosphate solubilization. Of two strains, B. altitudinis DT-89 function more prominently with respect to plant growth promotion and drought tolerance to plant in the early stage but protective traits of B. paramycoides DT-113 was more prominent after 75 days as evident by increased EPS (164%), root dry weight (144.44%), chlorophyll content (90.26%), SOD (389%) and proline (99.3%). The results support both the strains as a potential candidate to alleviate drought stress and enhance plant growth in the drought regions.

Keywords

Plant growth-promoting bacteria, Bacillus, EPS, Siderophore, IAA, GA

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