ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Md. Raihan Uddin1, Pranab Roy2 and Sukhendu Mandal1
1Department of Microbiology, Laboratory of Molecular Bacteriology, University of Calcutta, 35, Ballygunge Circular Road, Kolkata – 700 019, West Bengal, India.
2Department of Molecular Biology, Institute of Child Health, 11, Dr. Biresh Guha Street, Kolkata – 700 017, West Bengal, India.
J Pure Appl Microbiol. 2022;16(1):235-245 | Article Number: 7211
https://doi.org/10.22207/JPAM.16.1.13 | © The Author(s). 2022
Received: 31/07/2021 | Accepted: 16/12/2021 | Published online: 10/01/2022
Issue online: March 2022
Abstract

Polar microbiology remains as the most fascinating area of research which mainly focuses on exploration of psychrophilic organisms for having their cold-active enzymes of biotechnological potential. In this study, we have explored a culturable bacterial community and isolated 27 bacterial isolates with a different morphology from an unexplored site of Arctic region, for the possibility of identifying various active biomolecules. Screening of various isolates in a culture dependent manner helped us to identify strains capable of producing extracellular enzymes. The optimal growth parameters of most of the isolates are ranges between 18-22°C temperature, 3-5 days of incubation, 6-9 pH, and 3-5% (w/v) NaCl in LB media. It has also been found that among these isolates, 63% are able to produce lipase, 17% amylase, 7% xylanase and 7% isolates have responded for phosphatase activity but there are no isolates found for gelatinase and cellulase production ability. In addition, few isolates can also produce secretory protease, urease, β-galactosidase, etc. 16SrRNA gene sequence-based phylogeny revealed that the isolates belong to the genera of Psychrobacter, Planococcus, Halomonas, Arthrobacter, Oceanisphaera, Marinbacter, Pseudomonas, Algoriphagus. Strikingly, none of the Arctic isolates showed resistance towards commonly used antibiotics which indicates that the unexplored habitat is devoid of antibiotic exposure and so does the rise of antimicrobial resistance. The structure-function relationship of the isolated bioactive compounds from these isolates are the major focus of future research.

Keywords

Psychrotrophs, Arctic, Community Analysis, Polar Microbiology, Lipase, Pigment, Cold-active enzymes

Article Metrics

Article View: 52

Share This Article

© The Author(s) 2022. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.