ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Ririn Krisnawati1, Sardjono1, Jaka Widada2, Dian Anggraini Suroto1 and Muhammad Nur Cahyanto1
1Department of Food and Agricultural Product Technology, Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta – 55281, Indonesia.
2Department of Agricultural Microbiology, Faculty of Agricultural, Universitas Gadjah Mada, Yogyakarta – 55281, Indonesia.
J Pure Appl Microbiol. 2022;16(1):226-234 | Article Number: 7260
https://doi.org/10.22207/JPAM.16.1.12 | © The Author(s). 2022
Received: 19/09/2021 | Accepted: 03/12/2021 | Published online: 10/01/2022
strong>Issue online: March 2022
Abstract

Xylanases are widely produced by fungi, and the production of polysaccharide-degrading enzymes, in general, are usually subjected to carbon catabolite repression. In this work, the ability of several Indonesian indigenous fungi to produce endo-xylanase and β-xylosidase and their responses to glucose as a repressor were determined. Ten fungi were grown in a liquid medium supplemented with glucose as the repressor (0, 1%, 3%, and 5%), and the endo-xylanase and β-xylosidase productions were assayed. Aspergillus aculeatus FIG1 and A. oryzae KKB4 produced 3.85 and 0.70 U/mL of endo-xylanase, respectively, compared with other strains (0.22 U/mL or less). Trichoderma asperellum PK1J2, T. virens MLT2J2, A. aculeatus FIG1, T. asperellum MLT5J1, A. oryzae KKB4, and T. asperellum MLT3J2 produced 0.021–0.065 U/mL of β-xylosidase, whereas the other strains produced 0.013 U/mL or less of β-xylosidase. Adding 1% glucose to the growth medium can partially repress endo-xylanase production in A. aculeatus FIG1, T. asperellum PK1J2, and T. virens MLT4J1 and completely repress other strains. By adding 1% glucose, strains FIG1, PK1J2, and MLT4J1 suffered almost complete repression of β-xylosidase production, although such strains exhibited partial repression of endo-xylanase production. β-Xylosidase produced by the other strains showed complete repression by adding 1% glucose, except for A. aculeatus FIG1, A. tamarii FNCC 6151, and T. asperellum MLT1J1, which showed partial repression. Therefore, adding 3% glucose to the growth medium can result in complete repression of endo-xylanase and β-xylosidase productions in all strains examined.

Keywords

Endo-xylanase, β-xylosidase, carbon catabolite repression, glucose

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