ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Sonia Ambade1 , Neelima Deshpande2 and Pragati Abhyankar1
1Department of Microbiology, H.V. Desai College, Pune – 411 002, Maharashtra, India.
2Department of Microbiology, A.G. College, Pune – 411 004, Maharashtra, India.
J Pure Appl Microbiol. 2022;16(1):174-181 | Article Number: 7290 | © The Author(s). 2022
Received: 05/09/2021 | Accepted: 02/12/2021 | Published online: 07/01/2022
Issue online: March 2022

Mouthwash is one of the most convenient and effective method employed for dental plaque management. The aim of the undertaken study was to establish the antimicrobial and anti-biofilm properties of lemongrass essential oil (LGEO) based mouthwash on microbial flora from dental plaque and also check cytotoxicity of mouthwash formulation. Five main colonizers of dental plaque representing dental microflora and three different bacterial species mainly responsible for the formation of biofilm were selected in this study. LGEO based mouthwash was developed and its stability was also determined. The antimicrobial and anti-biofilm activity of LGEO based mouthwash has been evaluated against the representative dental microflora as per CLSI guidelines. Cytotoxicity of mouthwash was checked by globally used MTT assay employing NIH 3T3 mouse fibroblast cell line. The mouthwash has been found to exhibit the stability in its major component, citral and also found exhibit antimicrobial and anti-biofilm activity against dental microflora. No cytotoxic effect was observed on mouse fibroblast cell line. LGEO in formulated mouthwash being a natural, herbal material isolated from traditional medicinal plants appears as a good and effective substitute to control the microflora linked with dental plaque.


LGEO, Dental flora, Mouthwash, MTT Assay

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© The Author(s) 2022. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.