ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Swathy Krishna Jayalekshmi, Arya Radhakrishnan Krishna, Trisha Mary Pandipilly Antony and Suganthi Ramasamy
School of Biotechnology, Dr. G.R. Damodaran College of Science (Autonomous), Coimbatore – 641 014, Tamil Nadu, India.
J Pure Appl Microbiol. 2022;16(1):157-166 | Article Number: 7229 | © The Author(s). 2022
Received: 09/08/2021 | Accepted: 10/12/2021 | Published online: 03/01/2022
Issue online: March 2022

Foodborne pathogens are the main threat and cause of food poisoning. The majority of food infections have been related to the biofilm formation of foodborne pathogens in the food industry. Shewanella putrefaciens (KX355803, GRD 03), a Gram-negative pathogen isolated from mackerel fish, was identified and recognized as a food spoilage bacterium and a strong biofilm producer. The adhesion or attachment ability of Shewanella putrefaciens was determined on steel, plastic, glass, PVC and wood. NB (Nutrient broth), LB (Luria-Bertani broth), TSB (Tryptic soy broth) and BHI (Brain heart infusion broth) were enriched with glucose and shows optimum for bacterial adhesion. In the microtiter plate method (MTP), the strong attachment was observed at 48 and 72 hours of incubation and significant differences were obtained at p < 0.05. As the incubation period increases, the OD value (Optical density) of samples also increase. Biofilm formation is the major cause cross-contamination, and shows resistance to certain disinfectants, which leads to environmental stress tolerance. This study suggested with optimum biofilm production of isolate from fish by using glucose enriched media on different substrates, also comparing different growth media provide a detailed idea about biofilm-forming ability at different incubation time intervals.


Biofilm, Foodborne pathogen, Optical density, Disinfectant, Substrate

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© The Author(s) 2022. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.