ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Abderrahim Hatib1, Najwa Hassou1, Abdelouahab Benani2,Jamal Eddine Hafid3 and Moulay Mustapha Ennaji1
1Team of Virology, Oncology and Molecular Biotechnology, Laboratory of Virology, Microbiology, Quality and Biotechnologies/ Eco Toxicology and Biodiversity, Faculty of Science and Technology Mohammedia University Hassan II of Casablanca Morocco, PB 146 Yasmina Mohammedia-20650, Morocco.
2Laboratory of Molecular Biology, Institute Pasteur of Casablanca, Morocco.
3Bioresources Laboratory and Food Safety, Faculty of Sciences and Techniques-Gueliz, B.P 549, Av. Abdelkarim El-khattabi, Gueliz, University Cadi Ayyad, Marrakech, Morocco.
J Pure Appl Microbiol. 2021;15(4):2358-2366 | Article Number: 7150 | © The Author(s). 2021
Received: 29/06/2021 | Accepted: 29/10/2021 | Published: 22/11/2021

Viral outbreaks can result from the consumption of contaminated bivalve mollusks. However, despite the regulation related to enteric bacteria in food products, the consumption of raw and undercooked mollusks remains linked to viral epidemics in human populations. Real-time RT-PCR is a highly sensitive approach for detecting and quantifying enteric viruses, and after eliminating enzymatic amplification inhibitors from samples of interest, sensitive and specific tests, like real-time RT-PCR, can facilitate the detection and quantification of a wide range of viruses that are concentrated in mollusk digestive tissues. The aim of the present study was to evaluate the prevalence of Group-A rotaviruses in mussel (Mytilus edulis Linnaeus, 1758) specimens (n=576) collected downstream of the Oued El Maleh Estuary, which is along the coast of Mohammedia City in Morocco, using real-time RT-PCR. Rotavirus A RNA was detected in 37.5% (n=18) of the 48 sample batches, and viral loads ranged from 0.42×101  to 1.8603×104 genomic copies per g digestive tissue. Most (72.22%) of the positive samples were collected during the wet season (September-April), and the probability of detecting rotaviruses was significantly greater during the wet season than during the dry season (P<0.001). Monitoring Rotavirus A and similar viruses in shellfish may help prevent viral contamination and preserve public health.


Rotavirus, Molluscs, Gastroenteritis, Environment, Real-Time RT-PCR

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© The Author(s) 2021. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.