ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Akkaraphol Srichaisupakit1 , Peechanika Chopjitt2 and Anusak Kerdsin2
1Department of Microbiology, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.
 2Faculty of Public Health, Kasetsart University, Chalermprakiat Sakon Nakhon Province Campus, Sakon Nakhon 47000, Thailand.
J Pure Appl Microbiol. 2021;15(4):2347-2357 | Article Number: 7069
https://doi.org/10.22207/JPAM.15.4.59 | © The Author(s). 2021
Received: 31/05/2021 | Accepted: 26/10/2021 | Published: 22/11/2021
Abstract

Bacteriophage, a predator virus of bacteria, is an abundant biological entity in the biosphere. With ultimate applications in medicine and biotechnology, new phages are extensively being isolated and characterized. The objective of the present study was to characterize lytic bacteriophage vB_Pae-PA14 infecting Pseudomonas aeruginosa ATCC 27853 that was isolated from seawater in Thailand. vB_Pae-PA14 was subjected to whole genome phylogenetic analysis, host range test, biofilm test and characterization. Results showed that the phage belonged to a group of N4-like viruses, could infect P. aeruginosa isolates including carbapenem-resistant P. aeruginosa. The burst size of vB_Pae-PA14 was 86 plaque-forming unit/infected cells. Also, the phage showed a greater ability to control planktonic P. aeruginosa cells than the biofilm cells. Phage could withstand physical stresses especially the high salt concentration. In brief, lytic bacteriophage vB_Pae-PA14 infecting P. aeruginosa was isolated and characterized, which might be useful in further bacteriophage lytic applications.

Keywords

Bacteriophage, Lytic, Pseudomonas aeruginosa, N4-like viruses, Carbapenem-resistant Pseudomonas aeruginosa

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