ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Daniel Lantang and Arsyam Mawardi
Department of Biology, Faculty of Mathematics and Natural Sciences, Cenderawasih University, Jayapura 99351, Papua, Indonesia.
J Pure Appl Microbiol. 2021;15(4):2295-2301 | Article Number: 6702 | © The Author(s). 2021
Received: 12/10/2020 | Accepted: 19/08/2021 | Published: 17/11/2021

This research aims to analyze the level of similarity and diversity among local isolates of B. thuringiensis Auky Island Padaido District in Biak Numfor Regency with NCBI gene bank base, the basis of which is to obtain B. thuringiensis isolates from jayapura local isolates that can act as controllers of Anopheles mosquito larvae. Several steps in the research are 16s gene amplification, PCR product purification, cloning using pTA2 vectors and transformation into competent E. coli Zymo 5α cells, confirmation with PCR colonies, recombinant plasmid isolation, sequencing analysis and phylogenetic tree construction. The isolates of ABNP8, ABNP9, ABNP11, ABNP12 and ABNP18 have been detected as local isolates from in Auky Island Padaido District in Biak Numfor Papua Regency that have great potential as bioinsecticides, and capable of controlling and killing Anopheles mosquito larvae. Of the five isolates, ABNP8 isolates had unique diversity and characteristics and were different from the four other isolates. Based on the similarity analysis in the MEGA7 program, the similarity rate reached 84%. Its diversity can be seen from the uniqueness of the sequence and its position in different branching dendrograms.


Anopheles, Bacillus thuringiensis, Genetic diversity, Auky Island, local isolates, Larva controller

Article Metrics

Article View: 547

Share This Article

© The Author(s) 2021. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.