ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Suriya Tingthong, Pitchayaporn Suwanakood, Pongsak Rattanachaikunsopon and Jidapa Sangswan
Department of Biological Science, Faculty of Science, Ubon Ratchathani University, Ubon Ratchathani – 34190, Thailand.
J Pure Appl Microbiol. 2021;15(4):1963-1975 | Article Number: 7136
https://doi.org/10.22207/JPAM.15.4.18 | © The Author(s). 2021
Received: 26/06/2021 | Accepted: 13/09/2021 | Published: 01/10/2021
Abstract

Rice straw is a major agricultural waste that can be used as an alternative substrate to expensive raw materials for endoglucanases (CMCase) production by microorganisms. This study aimed to search for a microorganism having the potential to produce endoglucanase from rice straw. From compost samples, 40 bacterial colonies were isolated on carboxymethylcellulose (CMC) agar. Among them, 16 isolates showed a hydrolysis zone on a CMC agar plate with hydrolysis (HC) values ranging from 1.15±0.02 to 4.40±0.52. Based on hydrolysis zone diameter and HC value, isolates CP1, CP2 and CP3 were further examined for their CMCase production in CMC broth. According to CMCase production and stability, isolate CP1 was selected for further study. The optimal pH and temperature for CMCase production of isolate CP1 were 5 and 45 °C, respectively. When using pre-treated rice straw as a substrate for semi-solid-state fermentation, the highest CMCase activity of 0.142 ± 0.008 U/mL was obtained in a medium containing pre-treated rice straw of 60 g/L. The sequence alignment analysis and phylogenetic analysis suggested that the isolate CP1 was likely to be Streptomyces thermocoprophilus. The microorganism obtained from this study may be not only industrially important but also beneficial to the environment.

Keywords

Endoglucanases, Streptomyces thermocoprophilus, rice straw, actinomyces, semi-solid-state fermentation

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