ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Nguyen Hoang Khue Tu1,3 , Ngo Thi Thanh Thuy1,3, Doan Thi Thanh Vinh1,3, Nguyen Pham Quynh Anh1,3 and Ha Dieu Ly2,3
1Department of Biotechnology, School of Biotechnology, International University, Ho Chi Minh City, Vietnam.
2School of Medicine, Vietnam National University, Ho Chi Minh City, Vietnam.
3Vietnam National University, Ho Chi Minh City, Vietnam.
J Pure Appl Microbiol. 2021;15(3):1643-1654 | Article Number: 7191 | © The Author(s). 2021
Received: 20/07/2021 | Accepted: 11/08/2021 | Published: 26/08/2021

The ability of endophytic fungi to produce valuable bioactive compounds when surviving in medicinal herbs. Finding out endophytic fungi originated from Catharanthus roseus with antimicrobial, antioxidant and cytotoxicity activities is important for pharmaceutical development. The isolation was based on the morphology of fungi. Identification was performed by sequencing 18S rRNA that was compared with known genes using Blast search in the combination of phylogenic analysis by using Clustal W and PhyML in GenomeNet. For the antimicrobial test, the agar diffusion method was used. DPPH scavenging assay for the antioxidant activity determination by using spectrophotometry. The cytotoxicity test was carried out by Sulforhodamine B method. LC-MS was applied for predicting components. We isolated the Fusarium oxysporum F01 strain originated from Catharanthus roseus. The aqueous extracts showed inhibition against Staphylococcus aureus ATCC 25923, Pseudomonas aeruginosa ATCC 27853 (15.00 ± 0.50 mm), Serratia marcescens ATCC 14756 (9.00 ± 0.87 mm), Vibrio parahaemolyticus ATCC 17802 (15.50 ± 0.87 mm), Escherichia coli ATCC 25922 (12.50 ± 0.50 mm). The antioxidant activity of the extract obtained from the supernatant was determined with an IC50 value of about 11 µg/mL. The extract also showed cytotoxicity effect on liver cancer cell line (HepG2) and breast cancer cell line (MCF-7) with the percentage of inhibition of 84.47 ± 3.18 and 94.69 ± 1.59, respectively. LC-MS was used to point out the presence of pratol, a melanogenesis agent. The study provided more interesting information about Fusarium oxysporum F01 isolated in Catharanthus roseus grown in Vietnam, contributing to pharmaceutical sources in the world.


Fusarium oxysporum F01, Catharanthus roseus, antimicrobial and antioxidant activity, cytotoxicity, LC-MS

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© The Author(s) 2021. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.