ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
R. Kavitha and N. Damodharan
Department of Pharmaceutics, SRM College of Pharmacy, SRM Institute of Science and Technology, Kattankulathur – 603 203, Kanchipuram District, Tamil Nadu, India.
J Pure Appl Microbiol. 2021;15(3):1517-1526 | Article Number: 7016
https://doi.org/10.22207/JPAM.15.3.46 | © The Author(s). 2021
Received: 06/05/2021 | Accepted: 03/08/2021 | Published: 17/08/2021
Abstract

The current investigation was conducted to maximise the production of the natural anticancer drug from the microbe isolated from the marine soil sample of the Coromandel Coast of the Bay of Bengal region of India. Yellow to red colour pigmented microbes separated by crowd plate method. Bacteria are producing strong colour product subjected to future study. The isolated strains were detected based on biochemical, morphological, and genetic characteristics. Pigment formation was found to be influenced strongly by conditions of the environment. The water-insoluble pigment extracted by acidified methanol and showed maximum absorbance at 535nm. A statistical screening procedure was adopted to select the optimum condition to produce the pigment. The carbon, nitrogen, medium pH, growth condition temperature and revolution of agitation were screened using the response surface methodology statistical model. The near optimum conditions for the production medium were affected by the concentration of peanut, L-proline, percentage inoculum pH and incubation time. When these conditions were employed yield increased as two-fold as the concentration of prodigiosin 789 mg/l.

Keywords

Prodigiosin, Marine soil, Optimization, Microbial pigment

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