ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Noha G. Abdallah1 , Faten M. Ali1, Lamiaa A. Adel1, Ahmed M. Elkotb2 and Walaa A. Ibrahim1
1Department of Medical Microbiology and Immunology, Faculty of Medicine, Ain Shams University, Abbasya, Postal code – 11591, Cairo, Egypt.
2Department of Obstetric and Gynecology, Faculty of Medicine, Ain Shams University, Abbasya, Postal code – 11591, Cairo, Egypt.
J Pure Appl Microbiol. 2021;15(3):1490-1500 | Article Number: 7119
https://doi.org/10.22207/JPAM.15.3.43 | © The Author(s). 2021
Received: 20/06/2021 | Accepted: 05/08/2021 | Published: 12/08/2021
Abstract

Group B streptococcus (GBS) is a common cause of infections in pregnant females and non-pregnant adults with chronic diseases (such as diabetes and cancer), also it is the main reason of septicaemia and meningitis in infants. The aim of this study was to figure out how common GBS is in pregnant women, the antimicrobial sensitivity pattern of the isolated GBS colonies and check the presence of scpB and rib virulence genes in these isolates. We screened 203 pregnant women attending the Maternity Hospital of Ain Shams University using vaginal sampling. Isolation was done on CHROMagarTM Strep B and sheep blood agar plates then identified via colony characters, Gram stain, test for catalase production, Christie–Atkins–Munch-Petersen (CAMP) test, test for hippurate hydrolysis and latex agglutination test. This was followed by an antibiotic susceptibility test. Finally, Detection of scpB and rib virulence genes by conventional PCR was done. Our study detected that the prevalence rate of GBS in involved pregnant women was 11.33%. A statistically significant association between colonization and history of spontaneous abortion and preterm labor was observed. CHROMagar™ StrepB showed the same sensitivity of sheep blood agar with extensive effort to isolate suspected GBS colonies from blood agar. GBS was 100% sensitive to levofloxacin, linezolid, cefepime, ceftaroline and ceftriaxone. Also, it was highly sensitive to vancomycin (91.3%). Sensitivity to clindamycin, azithromycin, penicillin and ampicillin was (21.70%, 21.70%,47.80%, 47.80%) respectively. The least sensitivity of GBS was to erythromycin ( 8.7%). All isolates possessed the scpB gene (100%) while only 18 isolates (78.26%) had the rib gene.

Keywords

Group B streptococcus (GBS), CHROMagarTM StrepB, scpB, rib, virulence genes

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© The Author(s) 2021. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.